Effects of Prenatal Dexamethasone on the Rat Pituitary Gland and Gonadotropic Cells in Female Offspring
2015
Autori:
Ristić, NatašaSevers, Walter
Nestorović, Nataša
Jarić, Ivana
Manojlović-Stojanoski, Milica
Trifunović, Svetlana
Pendovski, Lazo
Milošević, Verica
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2016, Silverchair Publisher
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Glucocorticoids have a strong influence on growth and maturation of
fetal organ systems, but overexposure to exogenous glucocorticoids may
retard fetal growth and alter developmental processes in sensitive
tissues. The aim of this study was to specifically determine whether
prenatal exposure to dexamethasone (Dx) altered normal development and
function of pituitary gonadotropic cells in neonatal, infant and
peripubertal female offspring. On day 16 of pregnancy, rat dams received
1.0 mg Dx/kg body weight (BW) s.c., followed by 0.5 mg Dx/kg BW on days
17 and 18 of gestation. Control gravid females received the same volume
of saline. Female offspring were sacrificed on days 5, 16 and 38 after
delivery. The volume of the pituitary gland estimated using Cavalieri's
principle was significantly reduced (p < 0.05). Using a
fractionator-physical disector method, we found reduced total numbers of
follicle-stimulating hormone (FSH) and luteinizing hormone (LH) cells (p
< 0.05), accompanied by a decrease (p < 0.05) in serum concentrations of
FSH and LH, while the relative intensity of FSH and LH
immunofluorescence remained unchanged in neonatal, infant and
peripubertal female offspring prenatally exposed to Dx. The data
document that overexposure to Dx during fetal development evokes
developmental programming of the female reproductive system at the
pituitary cellular level, which may be associated with impaired
reproductive function. (C) 2016 S. Karger AG, Basel
Ključne reči:
Dexamethasone; Developmental programming; Female offspring; Follicle-stimulating hormone cells; Luteinizing hormone cells; Pituitary; StereologyIzvor:
Cells Tissues Organs, 2015, 201, 2, 148-158
DOI: 10.1159/000443987
ISSN: 1422-6421
PubMed: 26950885