Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)
2015
Аутори:
Cingel, AleksandarSavic, Jelena
Ćosić, Tatjana
Raspor, Martin
Ghalawenji, Nabil
Smigocki, Ann
Ninković, Slavica
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The evaluation of transgenic plants which is usually carried out under
controlled conditions in culture rooms and greenhouses can yield
valuable information about the influence of introduced genes on a
transgenic plant phenotype. However, an overall assessment of plant
performance can only be made by testing transgenic plants in the field
environment. Thus, the effects of pyramided rice cystatin genes OCI and
OCII on morphological parameters of transgenic potato cv. Desiree,
Dragacevka and Jelica lines were compared under in vitro, greenhouse,
and field conditions. All analyzed OC co-expressing transgenic lines
exhibited normal phenotype, both in vitro and in greenhouse conditions.
In the field environment, eight of nine OCI/OCII lines were similar to
the wild-type control plants in their general phenotypic appearance.
Yield parameters, such as tuber number and tuber weight for these
phenotypically normal OCI/OCII lines, were also comparable to the
controls. Only transgenic cv. Jelica line 4 plants exhibited slightly
reduced growth, atypical leaf morphology and, contrary to the plants of
other transgenic lines and untransformed controls, failed to flower.
However, despite the phenotypic and developmental changes under field
conditions, the OCI/OCII Jelica line 4 did not exhibit a significant
decrease in tuber yield. Stacking of OCI and OCII genes preserves
important attributes of the parental lines, confirming that this
approach could be suitable for improving agronomical traits in potato.
Кључне речи:
transgenic potato plants; rice cystatins; field performancesИзвор:
Archives of Biological Sciences, 2015, 67, 3, 957-964
DOI: 10.2298/ABS141201058C
ISSN: 1821-4339