Testosterone application decreases the capacity for ACTH and corticosterone secretion in a rat model of the andropause
2015
Аутори:
Ajdžanović, VladimirJarić, Ivana
Živanović, Jasmina
Filipović, Branko
Ristić, Nataša
Miler, Marko
Milošević, Verica
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2015 Elsevier GmbH.
Метаподаци
Приказ свих података о документуАпстракт:
The culminating phase of ageing in males-andropause is characterized by
enhanced activity of the hypothalamic-pituitary-adrenal axis and
frequent glucocorticoid excess. In parallel, free testosterone
deficiency provides the baseline hormonal milieu for the ageing male.
The aim of this study was to illustrate (using diverse microscopic and
biochemical methodologies) the effects of testosterone application on
the capacity for adrenocorticotropic hormone (ACTH) and corticosterone
secretion in a rat model of the andropause. Middle-aged Wistar rats were
divided into sham-operated (SO; n = 8), orchidectomized (Orx; n = 8) and
testosterone treated orchidectomized (Orx +T; n = 8) groups.
Testosterone propionate (5 mg/kg b.w./day) was administered for three
weeks, while SO and Orx groups received the vehicle alone. ACTH cells
and the adrenal cortex were stained using immuno-histochemical,
immuno-fluorescent and histochemical procedures. Circulating
concentrations of testosterone, estradiol, ACTH and corticosterone, as
well as the adrenal tissue corticosterone levels were measured by
immunoassays. Testosterone application led to increased (p < 0.05) serum
concentrations of sex steroids. Consequently, in Orx+T rats the ACTH
cell nuclei volume increased (p < 0.05) by 34\%, while the volume
density of ACTH cells and their relative intensity of fluorescence
decreased (p < 0.05) by 46\% and 21\%, respectively, in comparison with
the corresponding parameters in the Orx group. Testosterone also induced
vasodilatation in the adrenocortical zona fasciculata, and decreased (p
< 0.05) the ACTH concentrations and adrenal tissue corticosterone levels
by 38\% and 31\%, respectively, compared to the Orx group. In
conclusion, testosterone administration caused a decrease in the
capacity for ACTH and corticosterone secretion in a rat model of the
andropause. (C) 2015 Elsevier GmbH. All rights reserved.
Кључне речи:
Andropause; Rats; Testosterone; CorticosteroneИзвор:
Acta Histochemica, 2015, 117, 6, 528-535
DOI: 10.1016/j.acthis.2015.04.002
ISSN: 1618-0372