Ghrelin effects on midgut tissue antioxidative defense and glutathione S-transferase activity in Lymantria dispar (Lepidoptera)
2015
Autori:
Perić Mataruga, VesnaVlahović, Milena
Mrdaković, Marija
Matić, Dragana
Grčić, Anja
Filipović, Aleksandra
Ilijin, Larisa
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The aim of the study was to examine changes in Cu. Zn superoxide
dismutase (SOD), catalase (CAT), their gel electrophoresis profiles,
glutathione reductase (GR) activity, amount of glutathione (GSH), and
the activity of glutathione S-transferase (GST) -phase II
biotransformation enzyme in the midgut tissue of gypsy moth (Lymantria
dispar L. (Lepidoptera: Lymantriidae)) larvae after ghrelin treatment.
Four subpicomolar injections of ghrelin (0.3 pmol) or physiological
saline (control) were applied every 24 h. The SOD, CAT, GR, GST
activity, and amount of GSH were higher in the ghrelin-treated group
than in the control. Electrophoresis gel bands of SOD and CAT had higher
area and density in the treated group. The effects of ghrelin on the
antioxidative defense and GST activity in insects were detected for the
first time. The results provided evidence for possible application of
insects as simple model systems in future studies of the role of ghrelin
in the antioxidative protection of complex organisms.
Ključne reči:
Superoxide dismutase; catalase; glutathione reductase; glutathione; glutathione S-transferaseIzvor:
Turkish Journal of Biology, 2015, 39, 4, 618-623Finansiranje / projekti:
- Uticaj magnetnih polja i drugih sredinskih stresora na fiziološke odgovore i ponašanje različitih vrsta (RS-MESTD-Basic Research (BR or ON)-173027)
DOI: 10.3906/biy-1502-15
ISSN: 1300-0152