Effect of stab injury in the rat cerebral cortex on temporal pattern of expression of neuronal cytoskeletal proteins: An immunohistochemical study
2015
Аутори:
Lavrnja, IrenaSavić, Danijela
Parabucki, Ana
Dacic, Sanja
Laketa, Danijela
Peković, Sanja
Stojiljkovic, Mirjana
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Compelling evidence now points to the critical role of the cytoskeleton
in neurodegeneration. In the present study, using an immunohistochemical
approach, we have shown that cortical stab injury (CSI) in adult Wistar
rats significantly affects temporal pattern of expression of
neurofilament proteins (NFs). a major cytoskeleton components of
neurons, and microtubule-associated proteins (MAP2). At 3 days
post-injury (dpi) most of the NFs immunoreactivity was found in pyknotic
neurons and in fragmentized axonal processes in the perilesioned cortex.
These cytoskeletal alterations became more pronounced by 10 dpi. At the
subcellular level CSI also showed significant impact on NFs and MAP-2
expression. Thus, at 3 dpi most of the dendrites disappeared, while
large neuronal somata appeared like open circles pointing to membrane
disintegration. Conversely, at 10 dpi neuronal perikarya and a few new
apical dendrites were strongly labeled. Since aberrant NF
phosphorylation is a pathological hallmark of many human
neurodegenerative disorders, as well as is found after stressor stimuli,
the present results shed light into the expression of neurofilaments
after the stab brain injury. (C) 2014 Elsevier GmbH. All rights
reserved.
Кључне речи:
Neurofilaments; Cortical stab injury; Cytoskeleton; ImmunohistochemistryИзвор:
Acta Histochemica, 2015, 117, 2, 155-162
DOI: 10.1016/j.acthis.2014.12.004
ISSN: 1618-0372