CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR OTHER Pinus SPECIES IN Pinus nigra
2015
Authors:
Sarac, ZoricaAleksic, Jelena M.
Dodos, Tanja
Rajcevic, Nemanja
Bojović, Srđan
Marin, Petar D.
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Due to the current lack of nuclear microsatellites (simple sequence
repeats SSRs) specifically developed for Pinus nigra, an important
European coniferous species, we cross-species amplified 12 EST-SSRs
(expressed sequence tagged SSRs) developed for other Pinus species in P.
nigra in order to delineate loci which can be used for assessing levels
of genetic diversity and genetic structuring in this species. We
amplified these loci in individuals from seven populations from the
central Balkans representing four recognized infraspecific taxa of P.
nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica).
Contrary to expectations on high transferability of EST-SSRs into
related species, only three out of 12 tested loci were successfully
amplified in P. nigra, but they displayed lack/low levels of
polymorphism or generated multilocus amplification products. Thus, our
estimates on levels of genetic diversity (HE = 0.183) and genetic
differentiation (F-ST = 0.007) were based on variability of a single
locus harboring four alleles only and they should be taken with
cautions. Our study highlights the need for the development of
high-resolution molecular markers, such as co-dominant genic or genomic
SSRs or predominantly biallelic SNPs, or utilization of anonymous
dominant markers, such as AFLPs, for genotyping in P. nigra.
Keywords:
cross-species amplification; genetic diversity; Pinus nigra; the BalkansSource:
Genetika-Belgrade, 2015, 47, 1, 205-217Funding / projects:
- Micromorphological, phytochemical and molecular investigations of plants - systematic, ecological and applicative aspects (RS-MESTD-Basic Research (BR or ON)-173029)
- Plant Biodiversity of Serbia and the Balkans - assesment, sustainable use and protection (RS-MESTD-Basic Research (BR or ON)-173030)
DOI: 10.2298/GENSR1501205S
ISSN: 1820-6069