Evaluation of the Antigenotoxic Effects of the Royal Sun Mushroom, Agaricus brasiliensis (Higher Basidiomycetes) in Human Lymphocytes Treated with Thymol in the Comet Assay
2015
Аутори:
Radakovic, MilenaDjelic, Ninoslav
Stevanovic, Jevrosima
Soković, Marina
Radovic, Dejan
van Griensven, Leo J. L. D.
Stanimirovic, Zoran
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The aim of this investigation was to evaluate the possible protective
activity of Agaricus brasiliensis (=A. blazei sensu Murrill) ethanol
extract against thymol-induced DNA damage in human lymphocytes. Before
we studied the possible interaction of thymol and A. brasiliensis
extract, each component was tested in the comet assay. Thymol
significantly increased DNA damage in human lymphocytes at higher
concentrations (20, 50, 100, 150, and 200 mu g/mL), whereas no genotoxic
effect of A. brasiliensis ethanol extract was observed. In simultaneous
treatment with thymol (200 mu g/mL) and A. brasiliensis ethanol extract
(50, 100, 150, and 200 mu g/mL), the latter failed to reduce a
thymol-induced DNA damaging effect regardless of the applied
concentrations. To confirm that thymol induces DNA damage via reactive
oxygen species, we performed cotreatment with quercetin. Cotreatment
with quercetin (100 and 500 mu g/mL) significantly reduced DNA damage
caused by thymol (200 mu g/mL), indicating that thymol exhibits
genotoxicity mainly through induction of reactive oxygen species.