Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and Suppresses Expression of Inflammatory Mediators in the Rat Model of Brain Injury
2015
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Аутори:
Lavrnja, IrenaParabucki, Ana
Brkić, Predrag
Jovanović, Tomislav
Dacic, Sanja
Savić, Danijela
Pantić, Igor
Stojiljković, Mirjana
Peković, Sanja
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The exact mechanisms by which treatment with hyperbaric oxygen (HBOT)
exerts its beneficial effects on recovery after brain injury are still
unrevealed. Therefore, in this study we investigated the influence of
repetitive HBOT on the reactive astrogliosis and expression of mediators
of inflammation after cortical stab injury (CSI). CSI was performed on
male Wistar rats, divided into control, sham, and lesioned groups with
appropriate HBO. The HBOT protocol was as follows: 10 minutes of slow
compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10
minutes of slow decompression, once a day for 10 consecutive days. Data
obtained using real-time polymerase chain reaction, Western blot, and
immunohistochemical and immunofluorescence analyses revealed that
repetitive HBOT applied after the CSI attenuates reactive astrogliosis
and glial scarring, and reduces expression of GFAP (glial fibrillary
acidic protein), vimentin, and ICAM-1 (intercellular adhesion
molecule-1) both at gene and tissue levels. In addition, HBOT prevents
expression of CD40 and its ligand CD40L on microglia, neutrophils,
cortical neurons, and reactive astrocytes. Accordingly, repetitive HBOT,
by prevention of glial scarring and limiting of expression of
inflammatory mediators, supports formation of more permissive
environment for repair and regeneration.
Извор:
Mediators of Inflammation, 2015, 498405Финансирање / пројекти:
- Ћелијска и молекулска основа неуроинфламације: потенцијала циљна места за транслациону медицину и терапију (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
DOI: 10.1155/2015/498405
ISSN: 1466-1861