Composition, Antimicrobial and Antioxidant Properties of Endemic Species Ferulago macedonica Micevski \& E. Mayer
2015
Аутори:
Mileski, Ksenija S.Džamić, Ana M.
Ćirić, Ana
Ristic, Mihailo S.
Grujic, Slavica M.
Matevski, Vlado S.
Marin, Petar D.
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Ferulago macedonica Micevski \& E. Mayer (Apiaceae), an endemic species
from Macedonia, was evaluated for its essential oil composition and
biological activity for the first time. The essential oil composition
from inflorescence and aerial parts, analyzed by gas chromatography and
gas chromatography-mass spectrometry (GC and GC-MS), indicated a-pinene
(43.10\% and 22.78\%) and sabinene (26.73\% and 15.46\%) as the main
components. The antioxidant activities of extracts was determinated
spectrophotometrically using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The
strongest activity had methanol extracts of inflorescence and aerial
parts (IC50= 490 mg/mL and IC50= 630 mg/mL of solution, respectively).
According to 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid)
(ABTS) test, the most effective were ethanol and methanol extracts of F.
macedonica inflorescence (1.69 and 1.63 mg Vit.C/g dw). Total phenolic
and flavonoid contents evaluation indicated that their amounts were
ranged between 34.12-59.68 mg gallic acid (GA)/g dw for phenols and
9.08-23.73 mg quercetin hydrate (QE)/g dw for flavonoids. Antimicrobial
activity of extracts and essential oils was investigated by micro-well
dilution assay. Minimum inhibitory (MIC) and minimum
bactericidal/fungicidal concentrations (MBC/MFC) were defined. The
methanol extract of inflorescence has shown to be the strongest in both,
antibacterial (MICs= 4-8 mg/mL; MBCs= 9-13 mg/mL) and antifungal
activity (MICs= 2-12 mg/mL; MFCs= 6-18 mg/mL). Essential oil from
inflorescence and aerial parts of F. macedonica manifested stronger
inhibition on growth of pathogenic bacteria and fungy compared to tested
extracts and positive standards.