Strain differences in contact hypersensitivity reaction to dinitrochlorobenzene (DNCB) in rats
2015
Authors:
Popov Aleksandrov, AleksandraMirkov, Ivana
Demenesku, Jelena
Ninkov, Marina
Zolotarevski, Lidija
Kataranovski, Dragan S.
Kataranovski, Milena
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Genetic factors are among the most important determinants of
susceptibility to induction of allergic contact dermatitis. A limited
number of studies of experimental contact hypersensitivity (CHS) in
animals has shown differences in the severity of CHS; however, the
underlying mechanisms are unknown. In this study comparative analysis of
CHS to low and high dinitrochlorobenzene (DNCB) doses regimen of
sensitization/challenge in inbred Dark Agouti (DA) and Albino Oxford
(AO) rats was examined. Basic aspects of draining lymph node (dLN)
activity (cellularity, proliferation), proinflammatory (IFN-gamma,
IL-17) and anti-inflammatory (IL-10) cytokine gene expression and
production, as well as IL-12 and IL-23 subunits mRNA expression, were
examined in challenge and sensitization phase of CHS reaction. Lower
(compared to DA) intensity of CHS in AO rats was associated with lack of
(or negligible) dLN responses in challenge phase (ex vivo, hapten- or
IL-2-stimulated cell proliferation and proinflammatory cytokine mRNA and
production levels) but with lack of changes in IL-10 response. Less
pronounced dLN activity of sensitized animals of this strain was
observed as well. Higher proliferative activity and more pronounced
proinflammatory cytokine response during challenge and sensitization
phase suggest these activities as underlying mechanisms of higher
susceptibility of DA rats to CHS response to DNCB. (C) 2014 Elsevier
Ltd. All rights reserved.
Keywords:
Contact hypersensitivity; Dinitrochlorobenzene; Draining lymph node; Proliferation; CytokinesSource:
Food and Chemical Toxicology, 2015, 75, 94-103
DOI: 10.1016/j.fct.2014.11.010
ISSN: 1873-6351