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BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE LEVELS IN RAT SERUM
dc.creator | Laketa, Danijela | |
dc.creator | Savić, Jasmina | |
dc.creator | Bjelobaba, Ivana | |
dc.creator | Lavrnja, Irena | |
dc.creator | Vasić, Vesna | |
dc.creator | Stojiljković, Mirjana | |
dc.creator | Nedeljković, Nadežda | |
dc.date.accessioned | 2016-05-23T11:00:11Z | |
dc.date.available | 2016-05-23T11:00:11Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1452-8266 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2093 | |
dc.description.abstract | Background: Cortical stab injury (CSI) induces changes in the activity, expression and cellular distribution of specific ectonucleotidases at the injury site. Also, several experimentally induced neuropathologies are associated with changes in soluble ectonucleotidase activities in the plasma and serum, whilst various insults to the brain alter purine compounds levels in cerebrospinal fluid, but also in serum, indicating that insults to the brain may induce alterations in nucleotides release and rate of their hydrolysis in the vascular system. Since adenine nucleotides and adenosine regulate diverse cellular functions in the vascular system, including vascular tone, platelet aggregation and inflammatory responses of lymphocytes and macrophages, alterations of ectonucleotidase activities in the vascular system may be relevant for the clinical outcome of the primary insult. Methods: We explored ectonucleotidase activities using specific enzyme assays and determined adenine nucleotides concentrations by the UPLC method in the rat serum after cortical stab injury. Results: At 4-h post-injury, ATP and AMP hydrolysis increased by about 60\% and 40\%, respectively, while phosphodiesterase activity remained unchanged. Also, at 4-h postinjury a marked decrease in ATP concentration and more than 2-fold increase in AMP concentration were recorded. Conclusions: CSI induces rapid up-regulation of nucleotide catabolizing soluble ectonucleotidases in rat serum, which leads to the observed shift in serum nucleotide levels. The results obtained imply that ectonucleotidases and adenine nucleotides participate in the communication between the brain and the vascular system in physiological and pathological conditions and thereby may be involved in the development of various human neuropathologies. | en |
dc.description.sponsorship | Serbian Ministry of Education, Science and Technological Development Project {[}41014, 172023] | |
dc.language | English | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41014/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172023/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of Medical Biochemistry | |
dc.subject | ectonucleotidases | |
dc.subject | extracellular adenine nucleotides | |
dc.subject | brain injury | |
dc.subject | rat | |
dc.title | BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE LEVELS IN RAT SERUM | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Лаврња, Ирена Ц.; Бјелобаба, Ивана; Недељковиц, Надезда; Стојиљковиц, Мирјана; Васиц, Весна; Савиц, Јасмине; Лакета, Данијела; | |
dc.rights.holder | © Society of Medical Biochemists of Serbia | |
dc.citation.issue | 2 | |
dc.citation.volume | 34 | |
dc.identifier.doi | 10.2478/jomb-2014-0025 | |
dc.identifier.scopus | 2-s2.0-84925633777 | |
dc.identifier.wos | 000348895300008 | |
dc.citation.spage | 215 | |
dc.citation.epage | 222 | |
dc.type.version | publishedVersion | en |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/5638/2015.pdf |