Antiradical-antimicrobial activity and phenolic profile of pomegranate (Punica granatum L.) juices from different cultivars: a comparative study
2015
Аутори:
Lantzouraki, Dimitra Z.Sinanoglou, Vassilia J.
Zoumpoulakis, Panagiotis G.
Glamočlija, Jasmina
Ćirić, Ana
Soković, Marina
Heropoulos, George
Proestos, Charalampos
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Pomegranate juice (PJ) constituents have shown to exhibit
anticarcinogenic, antimicrobial, antioxidant and antiviral activities.
In the present study, the concentration of phenolic compounds and the
antiradical activity of PJs from the fruits of the two relatively new
Greek cultivars ``Persephone{''}and ``Porphiroyeneti{''}were determined
in comparison to the ``Wonderful{''}cultivar. Total phenolic content and
antiradical activity of the examined juices were found to vary in the
same decreasing manner as follows: ``Porphiroyeneti{''} >
``Wonderful{''} > ``Persephone{''}. The antimicrobial activity of PJs
was also determined, showing equal or higher effect than commercial
antimicrobial agents (streptomycin, ampicillin, bifonazole and
ketoconazole). All tested extracts demonstrated noteworthy antibacterial
activity with minimal inhibitory concentration ranging from 0.05 to 0.20
mg mL(-1) and minimal bactericidal concentration ranging from 0.10 to
0.40 mg mL(-1). Moreover, PJ extracts showed satisfactory fungistatic
(0.05-0.2 mg mL(-1)) and fungicidal (0.1-0.3 mg mL(-1)) activity against
all fungi tested. Among the cultivars tested, ``Porphiroyeneti{''}showed
slightly better antiradical and antimicrobial activity. In addition, a
GC-MS methodology was developed for the determination of the phenolic
profile of the extracts of PJ after different types of chemical
hydrolysis. Finally, an HPLC-PDA-ESI-MSn analysis was conducted for the
identification of the phenolic compounds in the extracts of PJ. In
total, more than 30 nonanthocyanidinic and more than 20 anthocyanidinic
compounds were identified. Our results confirm the functionality of
pomegranate juices and the potential applications of PJ extracts towards
novel products as food additives or preservatives.
Извор:
Rsc Advances, 2015, 5, 4, 2602-2614
DOI: 10.1039/c4ra11795f
ISSN: 2046-2069