Effects of genistein on stereological and hormonal characteristics of the pituitary somatotrophs in rats
2014
Autori:
Trifunović, SvetlanaManojlović-Stojanoski, Milica
Ajdžanović, Vladimir
Nestorović, Nataša
Ristić, Nataša
Jarić, Ivana
Milošević, Verica
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The hypothalamic-pituitary somatotropic system plays a pivotal role in
the regulation of physiological processes and metabolism, which is
modulated by gonadal steroids. Considering that genistein belongs to the
phytoestrogen family and acts via similar mechanisms to estrogens, the
present study was designed to demonstrate whether genistein modulates
the morphofunctional characteristic of somatotrophs {[}growth hormone
(GH) cells] in adult rats in comparison with the effects of estradiol.
In the study, the orchidectomized adult rats were used as an appropriate
model system for testing the effects of this hormone-like substance.
Changes in the pituitary somatotrophs were evaluated histologically and
stereologically, while GH level was determined biochemically. Using
immunolabelling and stereological methods, we showed that orchidectomy
(Orx) provoked the decrease of GH cell volume density. After estradiol
treatment of Orx rats, the most prominent change concerned the pituitary
relative intensity of GH fluorescence and circulating GH level, which
were elevated 77 \% and 4.7-fold, respectively. Clearly, in contrast to
orchidectomy, estradiol treatment enhanced the GH cells activity.
Genistein treatment increased pituitary weight and volume, GH cell
volume density, the total number of GH cells, and GH blood concentration
(1.3-fold) in comparison to the Orx group. Although identical tendencies
followed estradiol and genistein administration, the changes observed
after genistein treatment were milder compared to estradiol treatment.
Ključne reči:
Genistein; Estradiol; Somatotrophs; Histology; StereologyIzvor:
Endocrine, 2014, 47, 3, 869-877
DOI: 10.1007/s12020-014-0265-3
ISSN: 1559-0100