Ribavirin shows immunomodulatory effects on activated microglia
2014
Аутори:
Savić, DanijelaStojiljkovic, Mirjana
Lavrnja, Irena
Parabucki, Ana
Bjelobaba, Ivana
Nedeljkovic, Nadezda
Herdegen, Thomas
Peković, Sanja
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Ribavirin (RBV) is synthetic purine nucleoside analogue, licensed as
anti-viral drug that displays immunomodulatory actions on various immune
cells. Our previous ex vivo studies have demonstrated immunosuppressive
effects of RBV on reactive T-lymphocytes in experimental autoimmune
encephalomyelitis. Here, we examined the effects of RBV on inflammatory
response of microglia. RBV potency to down-regulate microglia
inflammatory response was assessed by measuring microglia cell body
size, and the production of nitric oxide (NO) and pro-and
anti-inflammatory cytokines. RBV exerted cytotoxic effects on
LPS-stimulated microglia, leaving non-stimulated microglia unaffected.
The exposure of activated microglia to RBV led to: decrease in the level
of NO as a result of decreased cell number, lower average cell surface,
the reduction of membrane ruffling, the suppression of interleukin-6
release and promoted interleukin-10 production. On the other hand, RBV
promoted LPS-induced interleukin-1 beta release. Our results imply that
RBV is a complex immunomodulator showing both anti-and pro-inflammatory
effects on activated microglia.
Кључне речи:
Cytokines; cytotoxicity; inflammation; nucleoside analogue; primary microgliaИзвор:
Immunopharmacology and Immunotoxicology, 2014, 36, 6, 433-441
DOI: 10.3109/08923973.2014.971962
ISSN: 1532-2513