Production of hairy root cultures of lettuce (Lactuca sativa L.)
2014
Authors:
Tadić, VojinMilošević, Snežana
Cingel, Aleksandar
Petrić, Marija
Trifunović-Momčilov, Milana
Antonić Reljin, Dragana
Jovan, Tadic
Subotić, Angelina
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by
inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs.
Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS.
Approximately in 96.7\% cvs. Nansen and in 91.2\% Ljubljanska ledenka
inoculated explants produced hairy root when they were incubated on
Murashige and Skoog (MS) half-strength medium without plant growth
regulators. A total of 54\% of all hairy root cultures expressed GUS
activity. Every hairy root represented an independent transformation
event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times
the biomass of control root). A PCR analysis of the genomic DNA
confirmed the presence of marker and target genes in 15 hairy roots
examined.
Keywords:
Genetic Transformation; Agrobacterium Rhizogenes; LettuceSource:
Central European Journal of Biology, 2014, 9, 12, 1196-1205Funding / projects:
- Development and application of biotechnological methods for obtaining healthy plants of ornamental species (RS-MESTD-Technological Development (TD or TR)-31019)
DOI: 10.2478/s11535-014-0351-9
ISSN: 1895-104X