Surface characterization, hemo-and cytocompatibility of segmented poly(dimethylsiloxane)-based polyurethanes
2014
Аутори:
Pergal, Marija V.Brkljačić, Jelena
Tovilović-Kovačević, Gordana
Jovančić, Petar
Pezo, Lato
Vasiljević-Radović, Dana
Djonlagić, Jasna
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Segmented polyurethanes based on poly(dimethylsiloxane), currently used
for biomedical applications, have sub-optimal biocompatibility which
reduces their efficacy. Improving the endothelial cell attachment and
blood-contacting properties of PDMS-based copolymers would substantially
improve their clinical applications. We have studied the surface
properties and in vitro biocompatibility of two series of segmented
poly(urethane-dimethylsiloxane)s (SPU-PDMS) based on hydroxypropyl-and
hydroxyethoxypropyl-terminated PDMS with potential applications in
blood-contacting medical devices. SPU-PDMS copolymers were characterized
by contact angle measurements, surface free energy determination
(calculated using the van Oss-Chaudhury-Good and Owens-Wendt methods),
and atomic force microscopy. The biocompatibility of copolymers was
evaluated using an endothelial EA.hy926 cell line by direct contact
assay, before and after pre-treatment of copolymers with multicomponent
protein mixture, as well as by a competitive blood-protein adsorption
assay. The obtained results suggested good blood compatibility of
synthesized copolymers. All copolymers exhibited good resistance to
fibrinogen adsorption and all favored albumin adsorption. Copolymers
based on hydroxyethoxypropyl-PDMS had lower hydrophobicity, higher
surface free energy and better microphase separation in comparison with
hydroxypropyl-PDMS-based copolymers, which promoted better endothelial
cell attachment and growth on the surface of these polymers as compared
to hydroxypropyl-PDMS-based copolymers. The results showed that SPU-PDMS
copolymers display good surface properties, depending on the type of
soft PDMS segments, which can be tailored for biomedical application
requirements such as biomedical devices for short-and long-term uses.
Кључне речи:
polyurethanes; siloxanes; surface free energy; cell adhesion; protein adsorptionИзвор:
Hemijska Industrija, 2014, 68, 6, 731-741Финансирање / пројекти:
- Синтеза и карактеризација нових функционалних полимера и полимерних нанокомпозита (RS-MESTD-Basic Research (BR or ON)-172062)
DOI: 10.2298/HEMIND141103082P
ISSN: 2217-7426