Structure and properties of thermoplastic polyurethanes based on poly(dimethylsiloxane): Assessment of biocompatibility
2014
Аутори:
Pergal, Marija V.Brkljačić, Jelena
Tovilović-Kovačević, Gordana
Ostojic, Sanja
Godevac, Dejan
Vasiljevic-Radovic, Dana
Djonlagic, Jasna
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Properties and biocompatibility of a series of thermoplastic
poly(urethane-siloxane)s (TPUSs) based on alpha,omega-dihydroxy ethoxy
propyl poly(dimethylsiloxane) (PDMS) for potential biomedical
application were studied. Thin films of TPUSs with a different PDMS soft
segment content were characterized by H-1 NMR, quantitative C-13 NMR,
Fourier transform infrared spectroscopy (FTIR), atomic force microscopy
(AFM), differential scanning calorimetry (DSC), dynamic mechanical
analysis (DMA), contact angle, and water absorption measurements.
Different techniques (FTIR, AFM, and DMA) showed that decrease of PDMS
content promotes microphase separation in TPUSs. Samples with a higher
PDMS content have more hydrophobic surface and better waterproof
performances, but lower degree of crystallinity. Biocompatibility of
TPUSs was examined after attachment of endothelial cells to the
untreated copolymer surface or surface pretreated with multicomponent
protein mixture, and by using competitive protein adsorption assay.
TPUSs did not exhibit any cytotoxicity toward endothelial cells, as
measured by lactate dehydrogenase and
3-{[}4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide assays.
The untreated and proteins preadsorbed TPUS samples favored endothelial
cells adhesion and growth, indicating good biocompatibility. All TPUSs
adsorbed more albumin than fibrinogen in competitive protein adsorption
experiment, which is feature regarded as beneficial for
biocompatibility. The results indicate that TPUSs have good surface,
thermo-mechanical, and biocompatible properties, which can be tailored
for biomedical application requirements by adequate selection of the
soft/hard segments ratio of the copolymers. (C) 2013 Wiley Periodicals,
Inc.
Кључне речи:
poly(urethane-siloxane) films; biocompatibility; cell adhesion; competitive protein adsorptionИзвор:
Journal of Biomedical Materials Research Part A, 2014, 102, 11, 3951-3964
DOI: 10.1002/jbm.a.35071
ISSN: 1552-4965