Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization
2014
Аутори:
Kostic, Ivana T.Ilic, Vesna Lj.
Đorđević, Verica B.
Bukara, Katarina M.
Mojsilovic, Slavko B.
Nedovic, Viktor A.
Bugarski, Diana S.
Veljovic, Dorde N.
Misic, Danijela M.
Bugarski, Branko M.
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The present study was aimed at investigating the effect of isolation
process-gradual hypotonic hemolysis on chosen parameters of the
erythrocyte membranes (ghosts) originating from bovine and porcine
slaughterhouse blood. The estimation of the gradual hypotonic hemolysis
as a drug loading procedure for the erythrocyte ghosts was performed as
well.
Based on the results derived from analysis of the osmotic properties of
the erythrocytes, the gradual hemolysis was performed with high volume
of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling
>90\% of hemolysis for both types of erythrocytes. Detailed insight into
ghosts' morphology by field emission-scanning electron microscopy
revealed a distortion from erythrocyte shape and an altered surface
texture with increased bilayer curvature for both samples. Compared to
erythrocytes, an average diameter of ghosts from both type of
erythrocytes decreased for only about 10\%. The reported unidispersity
of the isolated ghosts is of great importance for their potential
application as vehicles of active compounds. Gradual hemolysis did not
lead to substantial loss of cholesterol and membrane/cytoskeleton
proteins. This result indicated the ghosts' possibility to mimic the
chemical and structural anisotropic environment of in vivo cell
membranes, which is of significance for drug diffusion and partition
coefficients. Induced shift of phosphatidylserine to external surface of
the ghosts demonstrated their potential application as vehicles for
targeted drug delivery to cells of reticuloendothelial system. Ultra
high-performance liquid chromatography and Fourier transform infrared
spectroscopy revealed the presence of a drug model -
dexamethasone-sodium phosphate, and its interaction with structural
components in both types of erythrocyte ghosts. (C) 2014 Elsevier B.V.
All rights reserved.
Кључне речи:
Slaughterhouse blood; Gradual hypotonic hemolysis; Erythrocyte ghosts; Drug loadingИзвор:
Colloids and Surfaces B-Biointerfaces, 2014, 122, 250-259
DOI: 10.1016/j.colsurfb.2014.06.043
ISSN: 1873-4367