The effect of mitochondrial complex I inhibitor on longevity of short-lived and long-lived seed beetles and its mitonuclear hybrids
2014
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Mitochondria are suggested to play a central role in ageing and
evolution of longevity. Gradual decline in mitochondrial function during
ageing and concomitant increase in production of reactive oxygen species
(ROS) leads to oxidative damage of macromolecules and impairment of ATP
synthesis. To assess relationship between ageing and oxidative stress
resistance we exposed different longevity lines of the seed beetle
(Acanthoscelides obtectus) to four concentrations of tebufenpyrad,
mitochondrial complex I inhibitor. Complex I is one of main sites of ROS
production during normal respiration and its inhibition elevates
oxidative stress. Our results showed that 24 h of exposure to
tebufenpyrad decreased survival and post-stress longevity due to
increased baseline mortality. Higher resistance was recorded in beetles
from lines selected for late reproduction and extended longevity (L)
than in early reproducing beetles (E). Also, females were more resistant
than males. Since complex I is under dual genetic control, our second
aim was to disentangle relative contribution of nuclear and
mitochondrial genes to the variation in longevity. We used crossed
combinations of distinct mitochondrial and nuclear genotypes (E x L, L x
E) and compared them to control hybrids where mitochondrial genome was
``transplanted{''} onto the original background (E x E, L x L). Our
study revealed significant effect of nucleus, i.e. higher survival and
post-stress longevity in beetles harbouring L nucleus. Mitochondrion
effect was significant only within L nuclear background where E
mitochondrion gave advantage.
Кључне речи:
Acanthoscelides obtectus; Ageing; Longevity; Age-specific selection; Complex I inhibitor; Mitonuclear hybridsИзвор:
Biogerontology, 2014, 15, 5, 487-501
DOI: 10.1007/s10522-014-9520-5
ISSN: 1573-6768