Pulmonary Immune Responses to Aspergillus Fumigatus in Rats
2014
Autori:
Mirkov, IvanaEl-Muzghi, Amal Atia Mhfuod
Djokic Jelena
Ninkov, Marina
Popov Aleksandrov, Aleksandra
Glamočlija, Jasmina
Kataranovski, Milena
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Objective To evaluate immunologic mechanisms underlying Aspergillus
fumigatus pulmonary infections in immunocompetent Dark Agouti (DA) and
Albino Oxford (AO) rats recognized as being susceptible to some
inflammatory diseases in different manners.
Methods Lung fungal burden (quantitative colony forming units, CFU,
assay), leukocyte infiltration (histology, cell composition) and their
function (phagocytosis, oxidative activity, CD11b adhesion molecule
expression) and cytokine interferon-gamma (IFN-gamma) and interleukin-17
and -4 (IL-17 and IL-4) lung content were evaluated following infection
(intratracheally, 1x10(7) conidia).
Results Slower reduction of fungal burden was observed in AO rats in
comparison with that in DA rats, which was coincided with less intense
histologically evident lung cell infiltration and leukocyte recovery as
well as lower level of most of the their activities including
intracellular myeloperoxidase activity, the capacity of nitroblue
tetrazolium salt reduction and CD11b adhesion molecule expression
(except for phagocytosis of conidia) in these rats. Differential
patterns of changes in proinflammatory cytokine levels (unchanged levels
of IFN-gamma and transient increase of IL-17 in AO rats vs continuous
increase of both cytokines in DA rats) and unchanged levels of IL-4 were
observed.
Conclusion Genetically-based differences in the pattern of antifungal
lung leukocyte activities and cytokine milieu, associated with
differential efficiency of fungal elimination might be useful in the
future use of rat models in studies of pulmonary aspergillosis.
Ključne reči:
A. fumigatus; Rats; Lung immune responsesIzvor:
Biomedical and Environmental Sciences, 2014, 27, 9, 684-694
DOI: 10.3967/bes2014.104
ISSN: 0895-3988