Evaluation of single-cell gel electrophoresis data: Combination of variance analysis with sum of ranking differences
2014
Аутори:
Heberger, KarolyKolarevic, Stoimir
Kračun-Kolarević, Margareta
Sunjog, Karolina
Gacic, Zoran
Kljajic, Zoran
Mitric, Milena
Vukovic-Gacic, Branka
Тип документа:
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Specimens of the mussel Mytilus galloprovincialis were collected from
five sites in the Boka Kotorska Bay (Adriatic Sea, Montenegro) during
the period summer 2011-autumn 2012. Three types of tissue, haemolymph,
digestive gland were used for assessment of DNA damage. Images of
randomly selected cells were analyzed with a fluorescence microscope and
image analysis by the Comet Assay IV Image-analysis system. Three
parameters, viz, tail length, tail intensity and Olive tail moment were
analyzed on 4200 nuclei per cell type. We observed variations in the
level of DNA damage in mussels collected at different sites, as well as
seasonal variations in response. Sum of ranking differences (SRD) was
implemented to compare use of different types of cell and different
measure of comet tail per nucleus. Numerical scales were transferred
into ranks, range scaling between 0 and 1; standardization and
normalization were carried out.
SRD selected the best (and worst) combinations: tail moment is the best
for all data treatment and for all organs; second best is tail length,
and intensity ranks third (except for digestive gland). The differences
were significant at the 5\% level. Whereas gills and haemolymph cells do
not differ significantly, cells of the digestive gland are much more
suitable to estimate genotoxicity. Variance analysis decomposed the
effect of different factors on the SRD values. This unique combination
has provided not only the relative importance of factors, but also an
overall evaluation: the best evaluation method, the best data
pre-treatment, etc., were chosen even for partially contradictory data.
The rank transformation is superior to any other way of scaling, which
is proven by ordering the SRD values by SRD again, and by cross
validation. (C) 2014 Elsevier B.V. All rights reserved.
Напомена:
This is the peer reviewed version of the following article: Héberger K, Kolarević S, Kračun-Kolarević M, Sunjog K, Gačić Z, Kljajić Z, Mitrić M, Vuković-Gačić B. Evaluation of single-cell gel electrophoresis data: Combination of variance analysis with sum of ranking differences. Mutat Res Toxicol Environ Mutagen. 2014;771:15–22. https://doi.org/10.1016/j.mrgentox.2014.04.028
Кључне речи:
Ecogenotoxicity; Comet assay; Analysis of variance; Ranking; Fluorescence; MusselsИзвор:
Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 2014, 771, 15-22
DOI: 10.1016/j.mrgentox.2014.04.028
ISSN: 1879-3592