Ćelije mišjeg moždanog endotela ostvaruju različit uticaj na produkciju interferona-γ i interleukina-17 in vitro
Murine brain endothelial cells differently modulate interferon-γ and interleukin-17 production in vitro
Abstract:
Brain endothelial cells (BEC) are the major constituents of the blood-brain barrier (BBB), the structure that controls entrance of immune cells into CNS parenchyma. Our aim was to investigate the influence of BEC on production of IL-17 and IFN-γ-cytokines that are important for CNS inflammation. To that end, co-cultivations of the bEnd.3 brain endothelial cell line and lymph node cells (LNC) were performed, and gene expression and production of IL-17 and IFN-γ were determined. It was found that bEnd.3 cells inhibited expression and production of IFN-γ, but not of IL-17. Additionally, bEnd.3 cells also reduced production of the major IFN-γ-promoting cytokine - IL-12 - in LNC. The observed variation in modulation of pro-inflammatory cytokines by BEC could be of importance for the understanding of CNS inflammation.
Keywords:
CNS; cytokine; inflammation; bEnd.3; lymph node; concanavalin ASource:
Archives of Biological Sciences, 2009, 61, 1, 29-36Funding / projects:
- Fiziološka i farmakološka modulacija imunoinflamatornih i malignih bolesti (RS-MESTD-MPN2006-2010-143029)
- Mehanizmi urođene i stečene imunosti u autoimunskim bolestima i infekciji (RS-MESTD-MPN2006-2010-145066)
DOI: 10.2298/ABS0901029M
ISSN: 0354-4664