Production of guaianolides in Agrobacterium rhizogenes - transformed chicory regenerants flowering in vitro
2014
Authors:
Bogdanović, MilicaTodorović, Slađana
Banjanac, Tijana
Dragicevic, Milan B.
Verstappen, Francel W. A.
Bouwmeester, Harro J.
Simonović, Ana
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Chicory (Cichorium intybus L) is rich in bitter sesquiterpene lactones,
mainly guaianolides: lactucin, 8-deoxylactucin, lactupicrin and their
11(S),13-dihydroderivatives-compounds recognized for their antimicrobial
and anti-cancer effects. In vitro plant tissue culture, and particularly
Agro bacterium rhizogeries-generated hairy root (HR) cultures, have many
advantages as systems for production of valuable secondary metabolites.
Although chicory HRs grow better than control culture, having nearly 60
times greater fresh weight gain, they do not contain a higher content of
guaianolides than wild type (wt) roots. Thus we have established in
vitro system comprised of wt root and HR cultures, and wt and
transformed regenerated plants of the same age, in rosette and flowering
stage, in order to study the effects of transformation, organogenesis
and flowering on guaianolides production. Both regeneration and
flowering in vitro were spontaneous, so the results were not influenced
by exogenous growth regulators. Some of the transformed clones grew
better, but all flowered earlier in comparison to wt plants. Floral
transition increased guaianolides content in both roots and leaves of
transformed, but not of wt plants. Expression of RolC oncogene
correlated with floral transition and with guaianolides accumulation. We
propose A. rhizogenes transformed plants at the flowering stage as an
alternative source of free guaianolides, where, in contrast to HRs,
entire plants can be used for the extraction. (C) 2014 Elsevier B.V. All
rights reserved.
Keywords:
Sesquiterpene Lactones; Guaianolides; Hairy root; Cichorium intybus; Rol genes; FloweringSource:
Industrial Crops and Products, 2014, 60, 52-59
DOI: 10.1016/j.indcrop.2014.05.054
ISSN: 1872-633X