Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress
2014
Аутори:
Vukovic-Gacic, BrankaKolarevic, Stoimir
Sunjog, Karolina
Tomović, Jelena
Knežević-Vukčević, Jelena
Paunović, Momir
Gacic, Zoran
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Genotoxic response of freshwater mussels U. tumidus and U. pictorum to
environmental stress was studied using comet assay on hemocytes and gill
cells. The mussels were acclimated to controlled laboratory conditions
for 10 days, and then exposed at 4 sites in the Sava and Danube rivers
in the area of the city of Belgrade. Samples of each species were taken
after 7, 14, and 30 days of exposure. The mussels sampled immediately
after acclimation served as controls. Genotoxic response in both species
was induced earlier at sites receiving untreated wastewaters from the
city's main collectors (7 days), than at the site receiving only
domestic wastewaters from small settlements located upstream from the
city (30 days). There was a correlation between the comet tail intensity
values in tissues of exposed mussels and the concentrations of zinc,
copper, iron, and arsenic at the exposure sites. The genotoxic responses
in both tissues of U. pictorum and in hemocytes of U. tumidus were
similar, while U. tumidus gill cells failed to exhibit significant
genotoxic response at two sites. These findings, together with higher
mortality of U. tumidus at the most polluted sites, promote U. pictorum
as a model for genotoxicity monitoring in freshwater environments.
Кључне речи:
Unio pictorum; Unio tumidus; Comet assayИзвор:
Hydrobiologia, 2014, 735, 1, 221-231
DOI: 10.1007/s10750-013-1513-x
ISSN: 1573-5117