Pyramiding rice cystatin OCI and OCII genes in transgenic potato (Solanum tuberosum L.) for resistance to Colorado potato beetle (Leptinotarsa decemlineata Say)
2014
Аутори:
Cingel, AleksandarSavić, Jelena
Ćosić, Tatjana
Zdravković-Korać, Snežana
Momčilović, Ivana
Smigocki, Ann
Ninković, Slavica
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Proteinase inhibitors oryzacystatins I and II (OCI and OCII) have shown
potential in controlling pests that utilize cysteine proteinases for
protein digestion. In order to achieve an additive effect against
Colorado potato beetle (CPB, Leptinotarsa decemlineata Say) digestive
proteinases, we combined two individual OC genes in potato (Solanum
tuberosum L.) cultivars Desiree, Dragaevka and Jelica. The OC genes were
stacked into the potato genome by simultaneous co-transformation with
both OCI and OCII genes or by sequential re-transformation of an
OCI-expressing transgenic line with an OCII gene. Low constitutive and
high wound-induced transcript levels of both OCI and OCII genes,
directed by the inducible pin2 promoter, were detected in all doubly
transformed lines from all three cultivars. Both recombinant OCs,
detected by immunoblot, were in an active conformation as shown by their
strong papain inhibitory activity. Although no significant differences
in CPB larval mortality were observed when larvae fed on OCI/OCII
transformed or control potato foliage, expression of both OCI and OCII
in potato plants caused a reduction in CPB adult body weight and in
weight of foliage consumed, indicating an additive effect. Overall,
these results show that pyramiding of the OCI and OCII genes could be an
effective strategy for breeding cultivars exhibiting elevated levels of
resistance to CPB.
Кључне речи:
Gene pyramiding; Co-transformation; Re-transformation; Oryzacystatins; Potato; Colorado potato beetleИзвор:
Euphytica, 2014, 198, 3, 425-438
DOI: 10.1007/s10681-014-1119-z
ISSN: 0014-2336