Long-term fructose-enriched diet introduced immediately after weaning does not induce oxidative stress in the rat liver
2014
Аутори:
Brkljačić, JelenaGlban, Alhadi M.
Mijuskovic, Ana
Nikolić-Kokić, Aleksandra
Elaković, Ivana
Velickovic, Natasa
Matić, Gordana
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Increased fructose consumption is correlated with the rising prevalence
of obesity, metabolic syndrome, and type 2 diabetes. It is believed that
reactive oxygen species contribute to the development and progression of
metabolic disturbances, especially those associated with insulin
resistance. Dietary fructose produces both pro-oxidative and
antioxidative effects, depending upon the experimental conditions,
dosage, duration of treatment, and pathophysiological milieu. The
effects of fructose overconsumption on young populations, which have an
increased risk of developing metabolic disorders in adulthood, have not
been fully elucidated. We have previously shown that rats subjected to a
long-term fructose-enriched diet immediately after weaning display
impaired hepatic insulin sensitivity. In this study, we tested the
hypothesis that long-term fructose consumption induces alterations in
the redox setting of the liver. Starting from the 21st day afterbirth,
male Wistar rats were maintained for 9 weeks on a standard diet
(control) or a fructose-enriched diet that consisted of standard food
and 10\% fructose solution instead of drinking water. The expression and
activity of antioxidant enzymes as well as lipid peroxidation and
protein damage markers were measured. The results showed that a
fructose-enriched diet led to an increased expression of mitochondrial
manganese superoxide dismutase but did not affect antioxidant enzymes
activity, lipid peroxidation, thiol content, and the level of protein
oxidation. Therefore, our results suggest that the decrease in hepatic
insulin sensitivity that was previously observed in rats that were kept
on the same diet regime might be attributed to molecular mechanisms
other than redox disbalance. A possible fructose-related micronutrient
deficiency should be examined. (C) 2014 Elsevier Inc. All rights
reserved.
Кључне речи:
Antioxidant enzymes; Fructose-fed rat; Liver; Oxidative stress; Young ratsИзвор:
Nutrition Research, 2014, 34, 7, 646-652
DOI: 10.1016/j.nutres.2014.06.006
ISSN: 0271-5317