High variability in the tissue culture response of root-tips of Allium ascalonicum individuals and optimization of the regeneration procedure
2014
Аутори:
Tubić, LjiljanaAnackov, Goran
Milojević, Jelena
Ghalawenji, Nabil
Banjac, Nevena
Igić, Ružica
Zdravković-Korać, Snežana
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
This study assessed the shoot regeneration capacity of root-tips
isolated from single seed-derived individual plants, obtained from a
wild, open-pollinated micropopulation of shallot (Allium ascalonicum).
Considerable variation was observed in the regeneration capacities of
individual lines, ranging from 0.93 to 100 \%, and a mean bud number per
root explant between 0.09 and 20.67. One line was found to be superior,
and was chosen for protocol optimization, focusing on the 2,4-D/BA
ratio, duration of the CI phase and light conditions. The application of
the optimized protocol to other lines, selected for their variable
regeneration capacities, enhanced the process of regeneration and
shortened the time required to obtain healthy plantlets, even in
inferior lines. However, highly responsive lines remained superior,
indicating that differences at the individual level must not be
overlooked. The conditions employed in this study may serve as a base to
facilitate the application of molecular breeding methods in shallot.
Кључне речи:
Alliaceae; Allium ascalonicum L.; Plant regeneration; Root-tips; Shallot; Tissue cultureИзвор:
Plant Cell Tissue and Organ Culture, 2014, 118, 1, 101-110
DOI: 10.1007/s11240-014-0465-9
ISSN: 0167-6857