6-hidroksidopaminska lezija striatuma izaziva promene irnk za dopaminske receptore kod parkisoničnog pacova

Abstract

The effects of four-site intrastriatal 6-hydroxydopamine (6-OHDA) lesions were examined in adult male rats. Five days after the lesions the animals were checked for specific rotational behavior induced by middle dose of amphetamine and the results confirmed the effectiveness of the lesions. The RNAs from the striatum were isolated at different time points after the lesion, and the RT-PCR analyse were performed for the D1 and D2 receptor mRNA. The results show a decline in the D2 receptor mRNA level (40%) at 6 h and 24 h points while this change was not observed seven days after the lesion. In contrast, no statistically significant changes in the level of the D1 receptor mRNA after the lesion at any time point were found.

Ispitivani su efekti četiri ubodne 6-hidroksidopaminske (6-OHDA) lezije striatuma kod odraslih mužjaka pacova. Pet dana nakon lezije, životinje su testirane na specifično rotaciono ponaš anje pod uticajem srednje doze amfetamina i rezultati su potvrdili efikasnost lezije. RNK iz striatuma su izolovane u različitim vremenskim tačkama nakon lezije i urađena je RT-PCR analiza iRNK za D1 i D2 dopaminske receptore. Rezultati pokazuju smanjivanje nivoa iRNK za D2 receptor (40%) 6 h i 24 h nakon lezije, dok sedam dana nakon lezije nema promena. Za razliku od ovih rezultata, u nivou iRNK za D1 receptor ne postoje statistički značajne razlike u bilo kojoj vremenskoj tački.