dc.creator | Mijuskovic, Ana | |
dc.creator | Oreščanin Dušić, Zorana | |
dc.creator | Nikolić-Kokić, Aleksandra | |
dc.creator | Slavic, Marija | |
dc.creator | Spasić, Mihajlo | |
dc.creator | Spasojevic, Ivan | |
dc.creator | Blagojević, Duško | |
dc.date.accessioned | 2016-05-23T11:00:33Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1734-1140 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2206 | |
dc.description.abstract | Background: Our aim was to investigate the effect of methanethiol
(CH3SH) on contractility of rat uterus and activities of redox-active
enzymes, and to compare them with the effect of sodium sulphide (Na2S),
a hydrogen sulphide (H2S/HS-) donor.
Methods: Uteri were isolated from virgin Wistar rats, divided into six
groups, controls (untreated uteri allowed to contract spontaneously and
in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and
Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced).
Underlying antioxidative enzyme activities (superoxide dismutase - SOD,
glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or
Na2S-treated uteri were compared to controls.
Results: Our experiments showed that CH3SH and Na2S provoked reversible
relaxation of both spontaneous and Ca2+ induced uterine contractions.
The dose-response curves differed in shape, and CH3SH curve was shifted
to higher concentration compared to H2S/HS-. The effects of Na2S fitted
sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked
increased SOD activity and decreased GR activity. However, Na2S
(H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+
stimulated uteri, while the activity of GSHPx was increased in both
types of active uteri.
Conclusion: Our results imply that CH3SH may have a constructive role in
the control of muscle function and metabolism. Observed differences
between CH3SH and H2S/HS- could be attributed to a larger moiety that is
present in CH3SH compared to H2S, but they are more likely to be a
consequence of the specific actions of HS-, in relation to its negative
charge. (C) 2014 Institute of Pharmacology, Polish Academy of Sciences.
Published by Elsevier Urban \& Partner Sp. Z o.o.. All rights reserved. | en |
dc.description.sponsorship | Ministry of Science and Technological Development of the Republic of
Serbia {[}173014] | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | Pharmacological Reports | |
dc.subject | Methanethiol | |
dc.subject | Hydrogen sulfide | |
dc.subject | Relaxation | |
dc.subject | Reactive oxygen species | |
dc.title | Comparison of the effects of methanethiol and sodium sulphide on uterine
contractile activity | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Николић-Кокић, Aлександра; Благојевић, Душко П.; Мијусковиц, Aна; Орешчанин-Душић, Зорана С.; Славиц, Марија; Спасић, Михајло Б.; Спасојевиц, Иван; | |
dc.citation.issue | 3 | |
dc.citation.volume | 66 | |
dc.identifier.doi | 10.1016/j.pharep.2013.12.012 | |
dc.identifier.scopus | 2-s2.0-84901450234 | |
dc.identifier.wos | 000336948900005 | |
dc.citation.spage | 373 | |
dc.citation.epage | 379 | |
dc.type.version | publishedVersion | en |
dc.citation.rank | M23 | |