Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity
2014
Autori:
Mijuskovic, AnaOreščanin Dušić, Zorana
Nikolić-Kokić, Aleksandra
Slavic, Marija
Spasić, Mihajlo
Spasojevic, Ivan
Blagojević, Duško
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Background: Our aim was to investigate the effect of methanethiol
(CH3SH) on contractility of rat uterus and activities of redox-active
enzymes, and to compare them with the effect of sodium sulphide (Na2S),
a hydrogen sulphide (H2S/HS-) donor.
Methods: Uteri were isolated from virgin Wistar rats, divided into six
groups, controls (untreated uteri allowed to contract spontaneously and
in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and
Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced).
Underlying antioxidative enzyme activities (superoxide dismutase - SOD,
glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or
Na2S-treated uteri were compared to controls.
Results: Our experiments showed that CH3SH and Na2S provoked reversible
relaxation of both spontaneous and Ca2+ induced uterine contractions.
The dose-response curves differed in shape, and CH3SH curve was shifted
to higher concentration compared to H2S/HS-. The effects of Na2S fitted
sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked
increased SOD activity and decreased GR activity. However, Na2S
(H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+
stimulated uteri, while the activity of GSHPx was increased in both
types of active uteri.
Conclusion: Our results imply that CH3SH may have a constructive role in
the control of muscle function and metabolism. Observed differences
between CH3SH and H2S/HS- could be attributed to a larger moiety that is
present in CH3SH compared to H2S, but they are more likely to be a
consequence of the specific actions of HS-, in relation to its negative
charge. (C) 2014 Institute of Pharmacology, Polish Academy of Sciences.
Published by Elsevier Urban \& Partner Sp. Z o.o.. All rights reserved.
Ključne reči:
Methanethiol; Hydrogen sulfide; Relaxation; Reactive oxygen speciesIzvor:
Pharmacological Reports, 2014, 66, 3, 373-379
DOI: 10.1016/j.pharep.2013.12.012
ISSN: 1734-1140