Morpho-functional characteristics of rat fetal thyroid gland are affected by prenatal dexamethasone exposure
2014
Аутори:
Manojlović-Stojanoski, MilicaFilipović, Branko
Nestorović, Nataša
Šošić-Jurjević, Branka
Ristić, Nataša
Trifunović, Svetlana
Milošević, Verica
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2014 Elsevier Inc.
Метаподаци
Приказ свих података о документуАпстракт:
Thyroid hormones (TH) and glucocorticoids strongly contribute to the
maturation of fetal tissues in the preparation for extrauterine life.
Influence of maternal dexamethasone (Dx) administration on thyroid
glands morpho-functional characteristics of near term rat fetuses was
investigated applying unbiased stereology. On the 16th day of pregnancy
dams received 1.0 mg/Dx/kg/b.w., followed by 0.5 mg/Dx/kg/b.w. on the
17th and 18th days of gestation. The control females received the same
volume of saline. The volume of fetal thyroid was estimated using
Cavalieri's principle; the physical/fractionator design was applied for
the determination of absolute number of follicular cells in mitosis and
immunohistochemically labeled C cells; C cell volume was measured using
the planar rotator. The functional activity of thyroid tissue was
provided from thyroglobulin (Tg) and thyroperoxidase (TPO)
immunohistochemical staining. Applying these design-based modern
stereological methods it was shown that Dx treatment of gravid females
led to a significant decrease of fetal thyroid gland volume in 19- and
21-day-old fetuses, due to decreased proliferation of follicular cells.
The Tg and TPO immunohistochemistry demonstrated that intensive TH
production starts and continues during the examined period in control
and Dx-exposed fetuses. Under the influence of Dx the absolute number of
C cells was lower in both groups of near term fetuses, although
unchanged relation between the two populations of endocrine cells,
follicular and C cells suggesting that structural relationships within
the gland are preserved. In conclusion maternal glucocorticoid
administration at the thyroid gland level exerts growth-inhibitory and
maturational promoting effects in near term rat fetuses. (C) 2014
Elsevier Inc. All rights reserved.
Кључне речи:
Thyroid; Fetal development; C cells; Follicular cells; Dexamethasone; StereologyИзвор:
Steroids, 2014, 84, 22-29
DOI: 10.1016/j.steroids.2014.03.006
ISSN: 1878-5867