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Morpho-functional characteristics of rat fetal thyroid gland are affected by prenatal dexamethasone exposure
dc.creator | Manojlović-Stojanoski, Milica | |
dc.creator | Filipović, Branko | |
dc.creator | Nestorović, Nataša | |
dc.creator | Šošić-Jurjević, Branka | |
dc.creator | Ristić, Nataša | |
dc.creator | Trifunović, Svetlana | |
dc.creator | Milošević, Verica | |
dc.date.accessioned | 2016-05-23T11:00:33Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1878-5867 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2207 | |
dc.description.abstract | Thyroid hormones (TH) and glucocorticoids strongly contribute to the maturation of fetal tissues in the preparation for extrauterine life. Influence of maternal dexamethasone (Dx) administration on thyroid glands morpho-functional characteristics of near term rat fetuses was investigated applying unbiased stereology. On the 16th day of pregnancy dams received 1.0 mg/Dx/kg/b.w., followed by 0.5 mg/Dx/kg/b.w. on the 17th and 18th days of gestation. The control females received the same volume of saline. The volume of fetal thyroid was estimated using Cavalieri's principle; the physical/fractionator design was applied for the determination of absolute number of follicular cells in mitosis and immunohistochemically labeled C cells; C cell volume was measured using the planar rotator. The functional activity of thyroid tissue was provided from thyroglobulin (Tg) and thyroperoxidase (TPO) immunohistochemical staining. Applying these design-based modern stereological methods it was shown that Dx treatment of gravid females led to a significant decrease of fetal thyroid gland volume in 19- and 21-day-old fetuses, due to decreased proliferation of follicular cells. The Tg and TPO immunohistochemistry demonstrated that intensive TH production starts and continues during the examined period in control and Dx-exposed fetuses. Under the influence of Dx the absolute number of C cells was lower in both groups of near term fetuses, although unchanged relation between the two populations of endocrine cells, follicular and C cells suggesting that structural relationships within the gland are preserved. In conclusion maternal glucocorticoid administration at the thyroid gland level exerts growth-inhibitory and maturational promoting effects in near term rat fetuses. (C) 2014 Elsevier Inc. All rights reserved. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development of the Republic of Serbia {[}173009] | |
dc.language | English | |
dc.publisher | Elsevier | |
dc.rights | restrictedAccess | |
dc.source | Steroids | |
dc.subject | Thyroid | |
dc.subject | Fetal development | |
dc.subject | C cells | |
dc.subject | Follicular cells | |
dc.subject | Dexamethasone | |
dc.subject | Stereology | |
dc.title | Morpho-functional characteristics of rat fetal thyroid gland are affected by prenatal dexamethasone exposure | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Манојловић-Стојаноски, Милица; Трифуновиц, Светлана Л.; Шошић-Јурјевић, Бранка Т.; Филиповић, Бранко; Ристиц, Натаса М.; Несторовић, Наташа М.; Милосевиц, Верица Љ.; | |
dc.rights.holder | © 2014 Elsevier Inc. | |
dc.citation.volume | 84 | |
dc.identifier.doi | 10.1016/j.steroids.2014.03.006 | |
dc.identifier.scopus | 2-s2.0-84897545680 | |
dc.identifier.wos | 000336696700005 | |
dc.citation.spage | 22 | |
dc.citation.epage | 29 | |
dc.type.version | publishedVersion | en |