Quantification of arabinogalactan proteins during in vitro morphogenesis induced by beta-D-glucosyl Yariv reagent in Centaurium erythraea root culture
2014
Аутори:
Trifunović-Momčilov, MilanaTadić, Vojin
Petrić, Marija
Paunović, Danijela
Jevremović, Slađana
Subotić, Angelina
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Arabinogalactan proteins (AGPs) are a family of highly glycosylated cell
surface proteins located at the plasma membrane and plant cell wall.
AGPs play important roles in plant growth and development. Yariv
phenylglycoside (beta GlcY), synthetic red-brown dye that specifically
binds and precipitates AGPs, has been used for detection and
quantification of AGPs in plant tissue. Graded concentrations of beta
GlcY (0-75 mu M) were used to investigate the effect of this synthetic
dye on induction of in vitro morphogenesis in Centaurium erythraea root
culture on two nutrient media: A1/2MS and A1/2MS + IBA 1.0 mu M.
Regeneration of C. erythraea shoots on root explants was stimulated on
both media supplemented with 25 mu M beta GlcY after 8 weeks in culture.
Quantification of AGPs in different tissues of C. erythraea was
determinate with single radial diffusion method. This work emphasizes
clear effect of beta GlcY on induction of morphogenesis in vitro in C.
erythraea root culture.
Кључне речи:
Centaury; In vitro morphogenesis; Organogenesis; Hydroxyproline-rich glycoproteins; Single radial gel diffusionИзвор:
Acta Physiologiae Plantarum, 2014, 36, 5, 1187-1195
DOI: 10.1007/s11738-014-1495-y
ISSN: 1861-1664