The effects of dietary restriction and aging on amyloid precursor protein and presenilin-1 mRNA and protein expression in rat brain
2014
Аутори:
Mladenović, AleksandraPerovic, Milka
Smiljanić, Kosara
Todorovic, Smilja
Tesic, Vesna
Ruždijić, Sabera
Kanazir, Selma
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The objective of this study was to examine the effects of aging and
long-term dietary restriction (DR) on the level of amyloid precursor
protein (APP) and presenilin-1 (PS-1), proteins that are critically
involved in Alzheimer's disease. Changes in mRNA and protein expression
were assessed by real-time PCR and western blot analysis during aging
and long-term DR in the cortex and hippocampus of 6-, 12-, 18-, and
24-month-old rats. Prominent regional changes in expression were
observed in response to aging and DR. Although the hippocampus displayed
significant alterations in APP mRNA and protein expression and no
significant changes in PS-1 expression, an opposite pattern was observed
in the cortex. DR counteracted the age-related changes in APP mRNA
expression in both structures of old animals. The observed DR-induced
increase in mRNA levels in the hippocampus was accompanied by an
increase in the level of full-length protein APP. These results indicate
that although both structures are very sensitive to aging, a specific
spatial pattern of changes in APP and PS-1 occurs during aging.
Furthermore, these findings provide evidence that DR can affect APP and
PS-1 expression in a manner consistent with its proposed `antiaging'
effect.
Кључне речи:
rat; presenilin-1; cortex; Aging; dietary restriction; amyloid precursor protein; hippocampusИзвор:
Neuroreport, 2014, 25, 6, 398-403
DOI: 10.1097/WNR.0000000000000107
ISSN: 1473-558X