Calcium-induced alteration of mitochondrial morphology and mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes
2014
Аутори:
Golic, I.Velickovic, K.
Markelic, M.
Stancic, A.
Jankovic, A.
Vucetic, M.
Otašević, Vesna
Buzadžić, Biljana J.
Korać, Bato
Korac, A.
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Mitochondria are key organelles maintaining cellular bioenergetics and
integrity, and their regulation of {[}Ca2+](i) homeostasis has been
investigated in many cell types. We investigated the short-term
Ca-SANDOZ (R) treatment on brown adipocyte mitochondria, using imaging
and molecular biology techniques. Two-month-old male Wistar rats were
divided into two groups: Ca-SANDOZ (R) drinking or tap water (control)
drinking for three days. Alizarin Red S staining showed increased Ca2+
level in the brown adipocytes of treated rats, and potassium
pyroantimonate staining localized electron-dense regions in the
cytoplasm, mitochondria and around lipid droplets. Ca-SANDOZ (R)
decreased mitochondrial number, but increased their size and
mitochondrial cristae volume. Transmission electron microscopy revealed
numerous enlarged and fusioned-like mitochondria in the Ca-SANDOZ (R)
treated group compared to the control, and megamitochondria in some
brown adipocytes. The Ca2+ diet affected mitochondrial fusion as
mitofusin 1 (MFN1) and mitofusin 2 (MFN2) were increased, and
mitochondrial fission as dynamin related protein 1 (DRP1) was decreased.
Confocal microscopy showed a higher colocalization rate between
functional mitochondria and endoplasmic reticulum (ER). The level of
uncoupling protein-1 (UCP1) was elevated, which was confirmed by
immunohistochemistry and Western blot analysis. These results suggest
that Ca-SANDOZ (R) stimulates mitochondrial fusion, increases
mitochondrial-ER contacts and the thermogenic capacity of brown
adipocytes.
Кључне речи:
Brown adipocyte; mitochondrial dynamics; calcium; endoplasmic reticulumИзвор:
European Journal of Histochemistry, 2014, 58, 2377, 251-257
DOI: 10.4081/ejh.2014.2377
ISSN: 2038-8306