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Calcium-induced alteration of mitochondrial morphology and mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes
dc.creator | Golic, I. | |
dc.creator | Velickovic, K. | |
dc.creator | Markelic, M. | |
dc.creator | Stancic, A. | |
dc.creator | Jankovic, A. | |
dc.creator | Vucetic, M. | |
dc.creator | Otašević, Vesna | |
dc.creator | Buzadžić, Biljana J. | |
dc.creator | Korać, Bato | |
dc.creator | Korac, A. | |
dc.date.accessioned | 2016-05-23T11:00:41Z | |
dc.date.available | 2016-05-23T11:00:41Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 2038-8306 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2256 | |
dc.description.abstract | Mitochondria are key organelles maintaining cellular bioenergetics and integrity, and their regulation of {[}Ca2+](i) homeostasis has been investigated in many cell types. We investigated the short-term Ca-SANDOZ (R) treatment on brown adipocyte mitochondria, using imaging and molecular biology techniques. Two-month-old male Wistar rats were divided into two groups: Ca-SANDOZ (R) drinking or tap water (control) drinking for three days. Alizarin Red S staining showed increased Ca2+ level in the brown adipocytes of treated rats, and potassium pyroantimonate staining localized electron-dense regions in the cytoplasm, mitochondria and around lipid droplets. Ca-SANDOZ (R) decreased mitochondrial number, but increased their size and mitochondrial cristae volume. Transmission electron microscopy revealed numerous enlarged and fusioned-like mitochondria in the Ca-SANDOZ (R) treated group compared to the control, and megamitochondria in some brown adipocytes. The Ca2+ diet affected mitochondrial fusion as mitofusin 1 (MFN1) and mitofusin 2 (MFN2) were increased, and mitochondrial fission as dynamin related protein 1 (DRP1) was decreased. Confocal microscopy showed a higher colocalization rate between functional mitochondria and endoplasmic reticulum (ER). The level of uncoupling protein-1 (UCP1) was elevated, which was confirmed by immunohistochemistry and Western blot analysis. These results suggest that Ca-SANDOZ (R) stimulates mitochondrial fusion, increases mitochondrial-ER contacts and the thermogenic capacity of brown adipocytes. | en |
dc.description.sponsorship | Serbian Ministry of Education, Science and Technological Development {[}173055, 173054] | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | European Journal of Histochemistry | |
dc.subject | Brown adipocyte | |
dc.subject | mitochondrial dynamics | |
dc.subject | calcium | |
dc.subject | endoplasmic reticulum | |
dc.title | Calcium-induced alteration of mitochondrial morphology and mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Кораћ, Бато М.; Станциц, A.; Маркелиц, М.; Јанковиц, A.; Вуцетиц, М.; Корац, A.; Велицковиц, К.; Оташевић, Весна; Бузаджић, Биљана Ј.; Голиц, И.; | |
dc.citation.issue | 2377 | |
dc.citation.volume | 58 | |
dc.identifier.doi | 10.4081/ejh.2014.2377 | |
dc.identifier.scopus | 2-s2.0-84907579590 | |
dc.identifier.wos | 000347223200012 | |
dc.citation.spage | 251 | |
dc.citation.epage | 257 | |
dc.type.version | publishedVersion | en |
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