Tiazofurin modulates lipopolysaccharide-activated microglia in vitro
2014
Аутори:
Savić, DanijelaLavrnja, Irena
Dacic, Sanja
Bjelobaba, Ivana
Nedeljkovic, Nadezda
Stojiljkovic, Mirjana
Herdegen, Thomas
Peković, Sanja
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Tiazofurin is a purine nucleoside analogue, with a broad spectrum of
antitumoral and anti-inflammatory properties. In the present study, we
have investigated the effect of tiazofurin on microglial inflammatory
response to lipopolysaccharide in vitro. The cytotoxic effect of the
drug was examined by sulforhodamine B assay. The Griess method was used
to quantify nitrite production. Microglial morphology was assessed by
measuring cell body size. Release of the pro-inflammatory cytokines,
tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and the
anti-inflammatory cytokine interleukin-10, were evaluated by
enzyme-linked immunosorbent assay. Our data showed that tiazofurin
decreased the number of activated microglia, lowered nitric oxide
production and reduced the average cell surface of these cells.
Tiazofurin reduced tumor necrosis factor-alpha, interleukin-6 and
increased interleukin-10 secretion. Conversely, this drug promoted the
release of interleukin-1 beta. Results obtained in this study indicate
that TR displayed both anti-and pro-inflammatory modulation of activated
microglia that could be relevant for its antitumor action within the
central nervous system.
Кључне речи:
Tiazofurin; microglia; nitric oxide; cytokines; gliomaИзвор:
Archives of Biological Sciences, 2014, 66, 4, 1633-1640
DOI: 10.2298/ABS1404633S
ISSN: 1821-4339