Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause
2014
Аутори:
Ajdžanović, VladimirJarić, Ivana
Živanović, Jasmina
Šošić-Jurjević, Branka
Trifunović, Svetlana
Tanić, Nasta
Milošević, Verica
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Somatopause, the complex aspect of andropause, is recognizable by
reduced growth hormone - GH/insulin-like growth factor 1 axis function
in the ageing male. Soy isoflavones (usually genistein and daidzein),
which are known for their beneficial effects in the treatment of ageing
symptoms, are active in the pituitary, as well. The
iromuno-histomorphometric and fluorescent characteristics of pituitary
growth hormone secreting cells, in an animal model of andropause, were
examined after a treatment with genistein or daidzein. Andropausal
Wistar rats were divided into sham operated, orchidectomized and
genistein or daidzein treated orchidectomized groups. Genistein or
daidzein (30 mg/kg/day) were administered subcutaneously for three
weeks, while sham operated and orchidectomized groups received the
vehicle alone. Growth hormone secreting cells were identified by the
percoxidase-antiperoxidase immuno-histochemical, and inmuno-fluorescent
procedure. The main characteristic of growth hormone secreting cells in
soy isoflavones treated groups is a weaker immuno-histochemical staining
and immuno fluorescent signal compared to sham operated and
orchidectomized groups. The growth hormone secreting cell volume in
orchidectomized +genistein or +daidzein groups is by 13.8\% and 11.9\%
(p<0.05) smaller respectively, in comparison with the orchidectomized
group. In orchidectomized +genistein or +daidzein groups, the growth
hormone secreting cells relative volume density is by 62.5\% and 61.0\%
lower (p<0.05) respectively than for the sham operated group, and
decreased by 65.4\% and 64.0\% (p<0.05) respectively, compared to the
orchidectomized group. It can be concluded that chronic genistein or
daidzein treatment, in an animal model of andropause, attenuates
immuno-histomorphometric and fluorescent characteristics of growth
hormone secreting cells.
Кључне речи:
andropause; immuno-fluorescence; immuno-histochemistry; soy isoflavonesИзвор:
Acta Veterinaria-Beograd, 2014, 64, 1, 93-104
DOI: 10.2478/acve-2014-0010
ISSN: 1820-7448