dc.creator | Ristić, Nataša | |
dc.creator | Nestorović, Nataša | |
dc.creator | Manojlović-Stojanoski, Milica | |
dc.creator | Jarić, Ivana | |
dc.creator | Trifunović, Svetlana | |
dc.creator | Šošić-Jurjević, Branka | |
dc.creator | Milošević, Verica | |
dc.date.accessioned | 2016-05-23T11:00:45Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0065-1281 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2282 | |
dc.description.abstract | Overexposure to glucocorticoids during the fetal period induces changes
in developmental processes in various fetal tissues. The aim of this
study was to investigate the effects of the synthetic glucocorticoid,
dexamethasone (Dx), on pituitary volume and gonadotropic cells during a
critical period of pituitary development. The effects of Dx on
stereological parameters of the pituitary gland and FSH and LH cells
were investigated in 19 and 21-day old fetuses. On day 16 of pregnancy,
the experimental dams received 1.0 mg Dx/kg b.w. subcutaneously,
followed by 0.5 mg Dx/kg b.w./day on days 17 and 18 of gestation. The
control gravid females received the same volume of saline. FSH and LH
cells were stained immunohistochemically by the
peroxidase-antiperoxidase method (PAP). In 19-day old fetuses, exposure
to Dx caused a significant decrease of pituitary volume, estimated by
Cavalieri's principle. Also, the total number of FSH and LH cells per
pituitary, determined by physical fractionator counting technique, was
significantly reduced. These changes persisted until fetal day 21.
Volume densities and numerical densities of FSH and LH cells after
exposure to Dx in 19 and 21-day old fetuses remained unaffected. Our
results suggest that altered stereological parameters in pituitary gland
after exposure to dexamethasone in fetal period could be long-lasting.
(C) 2014 Elsevier GmbH. All rights reserved. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development of the
Republic of Serbia {[}173009] | |
dc.language | English | |
dc.publisher | Elsevier | |
dc.rights | restrictedAccess | |
dc.source | Acta Histochemica | |
dc.subject | Pituitary | |
dc.subject | Gonadotropic cells | |
dc.subject | Rat fetuses | |
dc.subject | Physical disector | |
dc.title | Exposure to dexamethasone reduces pituitary volume and gonadotropic cell
number in rat fetuses | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Трифуновиц, Светлана; Несторовић, Наташа М.; Ристиц, Натаса; Манојловић-Стојаноски, Милица; Медиговиц, Ивана; Шошић-Јурјевић, Бранка Т.; Милосевиц, Верица; | |
dc.rights.holder | © 2014 Elsevier GmbH. | |
dc.citation.issue | 5 | |
dc.citation.volume | 116 | |
dc.identifier.doi | 10.1016/j.acthis.2014.04.003 | |
dc.identifier.scopus | 2-s2.0-84904070410 | |
dc.identifier.wos | 000340011900044 | |
dc.citation.spage | 973 | |
dc.citation.epage | 980 | |
dc.type.version | publishedVersion | en |
dc.citation.rank | M23 | |