Activity of antioxidant enzymes during induction of morphogenesis of Fritillaria meleagris in bulb scale culture
2014
Аутори:
Petrić, MarijaJevremović, Slađana
Trifunović-Momčilov, Milana
Tadić, Vojin
Milosevic, Snezana
Subotić, Angelina
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
We investigated the activity of 3 antioxidant enzymes: superoxide
dismutase, catalase, and peroxidase during morphogenesis of Fritillaria
meleagris L. in vitro in bulb scale culture. Bulb cultures of F.
meleagris were established on nutrition medium supplemented with 1.0 mg
L-1 thidiazuron. Bulbs were grown at standard (24 degrees C) and low
temperatures (4 degrees C) or at an increased sucrose concentration
(4.5\%) during the first 6 weeks (pretreatments). All bulbs were then
grown for 4 weeks at standard temperature on 2,4-dichlorophenoxyacetic
acid and kinetin or thidiazuron (at concentrations of 1 mg L-1 each) in
order to examine the influence of the above-mentioned pretreatments and
nutrient medium composition on enzyme activity. Enzyme activity was
measured 7, 14, 21, and 28 days after the start of morphogenesis
induction. Superoxide dismutase and catalase showed the highest activity
at the beginning of morphogenesis in vitro. Peroxidase activity was the
highest in bulb segments immediately after isolation following
pretreatments and on both nutrition media. We also determined the effect
of pretreatment coupled with low temperature (4 degrees C) on growth and
development of F. meleagris in vitro and chlorophyll and carotenoids
content.
Кључне речи:
Snake's head fritillary; morphogenesis; superoxide dismutase; catalase; peroxidaseИзвор:
Turkish Journal of Biology, 2014, 38, 3, 328-338
DOI: 10.3906/biy-1309-45
ISSN: 1303-6092