Temperature-induced stress response in Lymantria dispar neurosecretory neurons
2014
Authors:
Ilijin, LarisaVlahović, Milena
Perić Mataruga, Vesna
Kmetič, Ivana
Grčić, Anja
Matić, Dragana
Mrdaković, Marija
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
The release of neurosecretory material from A2 neurosecretory neurons
(NSNs) was stimulated in Lymantria dispar fourth instar caterpillars
exposed to a temperature of 35 degrees C for 1, 12, and 24 h, as well as
those allowed recover after exposure (12 h at 35 degrees C, then 12 h at
23 degrees C). The levels of 2 protein forms with the same molecular
mass as bombyxin (3-4 and 4-5 kDa) increased with prolonged exposure to
35 degrees C. The second band was present only in the groups exposed to
this stressor. There was intensified synthetic activity and a low level
of secretion in L2' NSNs after exposure to 35 degrees C. We previously
found these NSNs to be immunopositive for prothoracicotropic
neurohormone. After this stress, densitometric analysis revealed a
decreased amount of the 11-12 kDa isoform (present in the control
group). The new isoform (13-15 kDa), expressed after exposure of the
insects to a high temperature, increased in amount with prolonged
exposure and after recovery at 23 degrees C. Short-term exposure of
caterpillars to high temperatures (35 degrees C) is a stressor and
activates carbohydrate metabolism, while PTTH immunopositive NSNs are
secretory-inactive during acute thermal stress regimes.
Keywords:
High temperature; Gypsy moth; Medial and lateral neurosecretory neuronsSource:
Turkish Journal of Biology, 2014, 38, 2, 157-167Funding / projects:
- The effects of magnetic fields and other environmental stressors on the physiological responses and behavior of different species (RS-MESTD-Basic Research (BR or ON)-173027)
DOI: 10.3906/biy-1306-75
ISSN: 1300-0152