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Regeneracija južno-jadranskog irisa somatskom embriogenezom
Plant regeneration of southern Adriatic iris by somatic embryogenesis
dc.creator | Jevremović, Slađana | |
dc.creator | Subotić, Angelina | |
dc.creator | Trifunović-Momčilov, Milana | |
dc.creator | Nikolić, Marija | |
dc.date.accessioned | 2015-08-28T10:26:51Z | |
dc.date.available | 2015-08-28T10:26:51Z | |
dc.date.issued | 2009 | sr |
dc.date.issued | 2009 | |
dc.identifier.issn | 0354-4664 | sr |
dc.identifier.other | Rad_konverzija_296 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/229 | |
dc.description.abstract | A simple protocol has been developed for plant regeneration by somatic embryogenesis of Southern Adriatic iris (Iris pseudopallida Trinajstić), an endemic species of the Balkan Peninsula. Somatic embryogenesis was induced in zygotic embryo culture on media supplemented with 2,4-dichlorophenoxy acetic acid (2-10 mgL-1) as the sole plant growth regulator, where both embryogenic calli and somatic embryos were induced. Subsequent decrease of 2,4-D in the media promoted formation of somatic embryos. Developed somatic embryos germinated on medium without growth regulators. The regenerated plantlets had diploid chromosome number. Planted plantlets acclimatized very well under greenhouse and garden conditions. | en |
dc.description.abstract | Jednostavan protokol za regeneraciju biljaka procesom somatske embriogeneze razvijen je za južno-jadranski iris (Iris pseudopallida Trinajstić), endemit Balkanskog poluostrva. Somatska embriogeneza je indukovana u kulturi 'zrelih' zigotskih embriona na hranljivoj podlozi obogaćenoj 2,4-dihlorofenoksi sirćetnom kiselinom (2,4-D), (kao jedinim regulatorom rastenja), gde su embriogeni kalus i somatski embrioni formirali. Smanjenje koncentracije 2,4-D u hranljivoj podlozi dovodi do povećanja broja formiranih somatskih embriona. Klijanje somatskih embriona je postignuto na podlozi bez regulatora rastenja. Regenerisane biljčice su imale diploidan broj hromozoma. Aklimatizacija biljaka je bila dobra u uslovima staklenika i bašte. | sr |
dc.description.sponsorship | Projekat ministarstva br. 142630 | sr |
dc.language.iso | eng | sr |
dc.rights | openAccess | sr |
dc.source | Archives of Biological Sciences | sr |
dc.subject | Zygotic embryo culture | ENG |
dc.subject | somatic embryogenesis | ENG |
dc.subject | Iris pseudopallida | ENG |
dc.title | Regeneracija južno-jadranskog irisa somatskom embriogenezom | sr |
dc.title | Plant regeneration of southern Adriatic iris by somatic embryogenesis | en |
dc.type | article | sr |
dc.rights.license | ARR | |
dcterms.abstract | Николић, Марија; Јевремовић, Слађана; Суботић, Aнгелина; Трифуновић-Момчилов, Милана; Регенерација јужно-јадранског ириса соматском ембриогенезом; Регенерација јужно-јадранског ириса соматском ембриогенезом; | |
dc.citation.issue | 3 | sr |
dc.citation.volume | 61 | sr |
dc.citation.spage | 413 | sr |
dc.citation.epage | 418 | sr |
dc.type.version | publishedVersion | sr |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs//bitstream/id/2341/Rad_konverzija_296.pdf | |
dc.citation.rank | M23 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_229 |