Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava
2014
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Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
In vivo (leaves and seed embryos) and in vitro (androgenic embryos)
antioxidant scavenging activity of Aesculus hippocastanum and Aesculus
flava medical plants was examined. Here we report antioxidant enzyme
activities of superoxide dismutase, catalase, guaiacol peroxidase and
glutathione peroxidase, reduced glutathione quantity, flavonoids,
soluble protein contents, quantities of malondialdehyde, and
(OH)-O-center dot radical presence in the investigated plant samples.
Total antioxidant capacity of all the samples of A. hippocastanum and A.
flava was determined using FRAP, DPPH, and NO center dot radical
scavenger capacity. The leaves of A. flava collected from the botanical
garden exhibited stronger antioxidant activity (higher activities of
SOD, and higher quantities of GSH, TSH, TPC, and scavenging abilities of
DPPH and NO center dot, and higher FRAP values and lowest quantities of
(OH)-O-center dot and MDA) than in vitro obtained cultures. However, the
leaves of A. flava showed higher antioxidant activity than the leaves of
A. hippocastanum, and therefore they have a stronger tolerance of
oxidative stress. Androgenic embryos of both species had low amount of
antioxidants due to controlled in vitro environmental conditions (T,
photoperiod, humidity, nutritive factors, and pathogen-free). Our
results confirmed that we found optimal in vitro conditions for
producing androgenic embryos of both Aesculus species. Also, we assume
that horse chestnut androgenic embryos can be used as an alternative
source for large-scale aescin production.
Извор:
Scientific World Journal, 2014, 767392Финансирање / пројекти:
- Биосенсинг технологије и глобални систем за континуирана истраживања и интегрисано управљање екосистемима (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43002)
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
DOI: 10.1155/2014/767392
ISSN: 1537-744X
PubMed: 24672369