Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles
2014
Аутори:
Banjanac, TijanaŠiler, Branislav
Skorić, Marijana
Ghalawenji, Nabil
Milutinović, Milica
Božić, Dragana
Misic, Danijela
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Most of the species from the genus Centaurium Hill readily hybridize
between themselves in natural habitats. Artificial interspecific hybrid
plants were obtained under in vitro conditions using reciprocal crosses
between 4 species: Centaurium erythraea, C. littorale, C. maritimum, and
C. pulchellum. Among 76 crossing experiments performed, 3 types of
crosses produced seeds and gave 20 interspecific hybrid plants. Nuclear
DNA quantification, fingerprinting of molecular markers, and
determination of variation in main secondary metabolite content were
employed to confirm their hybrid nature. All 3 approaches were capable
of detecting hybrid plants. RAPD molecular markers proved to be the most
reliable, always placing the hybrids between their parents in
dendrograms, while secoiridoid glycoside content also showed
intermediate profiles in the hybrid plants in relation to the parents.
Hence, each of the techniques employed can be recommended for
elucidating the mechanisms of interspecific hybridization in natural
populations of Centaurium species in further studies. This could help to
comprehend their breeding system and recognize interspecific
hybridization as one of the crucial steps in polyploid formation and
speciation in this genus.
Кључне речи:
Centaurium; interspecific hybridization; molecular markers; ploidy level; secoiridoid glycosidesИзвор:
Turkish Journal of Botany, 2014, 38, 1, 68-79
DOI: 10.3906/bot-1211-58
ISSN: 1300-008X