Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
2015
Аутори:
Bolic, BojanaMijuskovic, Ana
Popovic-Bijelic, Ana
Nikolić-Kokić, Aleksandra
Spasic, Snezana
Blagojević, Duško
Spasić, Mihajlo
Spasojevic, Ivan
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling
species, with superoxide dimutases (SOD) are poorly understood. We
applied low-T EPR spectroscopy to examine the effects of HS-/H2S and
superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and
CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD
and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while
manganese appears to be released from MnSOD active center. Untreated and
O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems,
i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of
(patho)physiological effects of HS-/H2S. (C) 2015 Elsevier Inc. All
rights reserved.
Кључне речи:
Superoxide dismutase; Iron; Manganese; CopperИзвор:
Nitric Oxide-Biology and Chemistry, 2015, 51, 19-23
DOI: 10.1016/j.niox.2015.09.008
ISSN: 1089-8611