Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity
2015
Аутори:
Todorovic, AnaPejic, Snezana
Stojiljkovic, Vesna
Gavrilovic, Ljubica
Popovic, Natasa
Pavlovic, Ivan
Saičić, Zorica
Pajovic, Snezana B.
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Чланак у часопису (Објављена верзија)
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Purpose Previously, we examined manganese superoxide dismutase (MnSOD),
copper-zinc superoxide dismutase (CuZnSOD), and catalase (CAT)
activities in rat brain irradiated with 2 or 3 Gy of gamma-rays. The
results indicated that lower MnSOD activity and inducibility found in
hippocampus might explain higher radiosensitivity of this brain region.
Thus, in this study, we wanted to determine changes of MnSOD, CuZnSOD,
and CAT activities after dose of 5 Gy and to find out if differences in
MnSOD activity are caused by changes in its expression.
Heads of 4-day-old female rats were irradiated with gamma-rays, using
Co-60. Animals were sacrificed 1/24 h after exposure. Hippocampus and
cortex tissues were prepared for enzyme activity measurements and
Western blot analysis.
One hour after exposure, gamma-rays significantly decreased MnSOD
activity in both examined brain regions. Twenty-four hours later, MnSOD
recovery showed dose and regional dependence. It was weaker at higher
doses and in hippocampal region. MnSOD expression changed in the similar
manner as MnSOD activity only at lower doses of gamma-rays. In both
examined brain regions, gamma radiation significantly decreased CuZnSOD
activity and did not change activity of CAT.
Our results confirmed that MnSOD plays an important role in different
regional radiosensitivity but also showed that depending on dose,
radiation affects MnSOD level by utterly different mechanisms.
Postradiation changes of CuZnSOD and CAT are not regionally specific and
therefore, cannot account for the different radiosensitivity of the
hippocampus and cortex.
Кључне речи:
Radiosensitivity; Brain; Hippocampus; CortexИзвор:
Childs Nervous System, 2015, 31, 12, 2249-2256
DOI: 10.1007/s00381-015-2807-2
ISSN: 1433-0350