dc.creator | Šošić-Jurjević, Branka | |
dc.creator | Filipović, Branko | |
dc.creator | Renko, Kostja | |
dc.creator | Miler, Marko | |
dc.creator | Trifunović, Svetlana | |
dc.creator | Ajdžanović, Vladimir | |
dc.creator | Koehrle, Josef | |
dc.creator | Milošević, Verica | |
dc.date.accessioned | 2016-05-23T11:00:53Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0531-5565 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2333 | |
dc.description.abstract | We previously reported that orchidectomy (Orx) of middle-aged rats
(15-16-month-old; MA) slightly affected pituitary-thyroid axis, but
decreased liver deiodinase (Dio) type I and pituitary Dio2 enzyme
activities. At present, we examined the effects of subsequent
testosterone-propionate treatment (5 mg/kg; Orx + T), and compared the
effects of testosterone with the effects of estradiol-clipropionate
(0.06 mg/kg; Orx + E) treatment. Hormones were subcutaneously
administered, daily, for three weeks, while Orx and sham-operated (SO)
controls received only the vehicle. The applied dose of T did not alter
serum TSH, T4 and T3 concentrations in Orx- MA, though it increased TSH
when administrated to Orx young adults (2.5-month-old; Orx-YA). However,
pituitaries of Orx-MA + T rats had higher relative intensity of
immunofluorescence (RIP) for TSHI3; in their thyroids we found increased
volume and height of follicular epithelium, decreased volume of the
colloid and higher RIF for T,{[}-bound to thyroglobulin (Tg-T4). Liver
Diol activity was increased. E-treatment did not affect serum hormone
levels, pituitary RIF for TS1-11i, or liver Diol activity in Orx-MA
rats. Thyroids had decreased relative volume and height of follicular
epithelium, increased relative volume of the colloid, decreased volume
of sodium iodide symporter-immunopositive epithelium and lower RIF for
Tg-T4. Detected changes were statistically significant. In conclusion,
androgenization enhanced pituitary TSHr, RIF, thyroid activation and
liver Diol enzyme activity in Orx-MA, without elevating serum TSH as in
Orx-YA rats. Estrogenization induced pituitary enlargement with no
effect on pituitary TSHI) RIF, serum TSH or liver Diol activity. E also
induced alterations in thyroid histology that indicate mild suppression
of its functioning, and contributed to thyroid blood vessel enlargement
in Orx-MA rats. (C) 2015 Elsevier Inc. All rights reserved. | en |
dc.description.sponsorship | Ministry of Education and Science of the Republic of Serbia {[}173009];
Deutsche Forschungsgemeinschaft {[}DFG-GK 1208, TP3, RE3038/1-1];
Charite Universitatsmedizin Berlin; European Society for Endocrinology
(ESE) | en |
dc.language | English | |
dc.publisher | Elsevier | |
dc.rights | restrictedAccess | |
dc.source | Experimental Gerontology | |
dc.subject | Testosterone | |
dc.subject | Estracliol | |
dc.subject | Pituitary-thyroid axis | |
dc.subject | Middle age | |
dc.subject | Rat | |
dc.title | Testosterone and estradiol treatments differently affect
pituitary-thyroid axis and liver deiodinase 1 activity in
orchidectomized middle-aged rats | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Aјджановић, Владимир; Шошић-Јурјевић, Бранка Т.; Милосевиц, В.; Коехрле, Ј.; Трифуновиц, С.; Милер, М.; Ренко, К.; Филиповиц, Б.; | |
dc.rights.holder | © 2015 Elsevier Inc. | |
dc.citation.volume | 72 | |
dc.identifier.doi | 10.1016/j.exger.2015.09.010 | |
dc.identifier.scopus | 2-s2.0-84942606456 | |
dc.identifier.wos | 000364989100012 | |
dc.citation.spage | 85 | |
dc.citation.epage | 98 | |
dc.type.version | publishedVersion | en |
dc.citation.rank | M21 | |