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Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes
dc.creator | Trikka, Fotini A. | |
dc.creator | Nikolaidis, Alexandros | |
dc.creator | Ignea, Codruta | |
dc.creator | Tsaballa, Aphrodite | |
dc.creator | Tziveleka, Leto-Aikaterini | |
dc.creator | Ioannou, Efstathia | |
dc.creator | Roussis, Vassilios | |
dc.creator | Stea, Eleni A. | |
dc.creator | Božić, Dragana | |
dc.creator | Argiriou, Anagnostis | |
dc.creator | Kanellis, Angelos K. | |
dc.creator | Kampranis, Sotirios C. | |
dc.creator | Makris, Antonios M. | |
dc.date.accessioned | 2016-05-23T11:00:53Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1471-2164 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2335 | |
dc.description.abstract | Background: Salvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a constant supply and developing biotechnological methods for their production could provide a sustainable alternative. The transcriptome of S. pomifera glandular trichomes was analysed aiming to identify genes that could be used in the engineering of synthetic microbial systems. Results: In the present study, a thorough metabolite analysis of S. pomifera leaves led to the isolation and structure elucidation of carnosic acid-family metabolites including one new natural product. These labdane diterpenes seem to be synthesized through miltiradiene and ferruginol. Transcriptomic analysis of the glandular trichomes from the S. pomifera leaves revealed two genes likely involved in miltiradiene synthesis. Their products were identified and the corresponding enzymes were characterized as copalyl diphosphate synthase (SpCDS) and miltiradiene synthase (SpMilS). In addition, several CYP-encoding transcripts were identified providing a valuable resource for the identification of the biosynthetic mechanism responsible for the production of carnosic acid-family metabolites in S. pomifera. Conclusions: Our work has uncovered the key enzymes involved in miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The transcriptomic dataset obtained provides a valuable tool for the identification of the CYPs involved in the synthesis of carnosic acid-family metabolites. | en |
dc.description.sponsorship | General Secretariat of Research and Technology (GSRT) {[}09-SYN-23-879]; grant SEE-ERA. NET PLUS {[}ERA 64/01]; grant KRIPIS {[}MIS 448840] | |
dc.language | English | |
dc.relation | General Secretariat of Research and Technology (GSRT) grant 09-SYN-23-879, grant SEE-ERA. NET PLUS: ERA 64/01 and grant KRIPIS (grant no. MIS 448840) | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Bmc Genomics | |
dc.subject | Miltiradiene | |
dc.subject | S. pomifera | |
dc.subject | Diterpenes | |
dc.subject | Cytochrome P450 | |
dc.title | Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes | en |
dc.type | article | |
dc.rights.license | BY | |
dcterms.abstract | Трикка, Фотини A.; Иоанноу, Ефстатхиа; Роуссис, Вассилиос; Тзивелека, Лето-Aикатерини; Тсабалла, Aпхродите; Стеа, Елени A.; Aргириоу, Aнагностис; Канеллис, Aнгелос К.; Кампранис, Сотириос Ц.; Макрис, Aнтониос М.; Божић, Драгана; Игнеа, Цодрута; Николаидис, Aлеxандрос; | |
dc.rights.holder | © 2015 Trikka et al | |
dc.citation.issue | 935 | |
dc.citation.volume | 16 | |
dc.identifier.doi | 10.1186/s12864-015-2147-3 | |
dc.identifier.scopus | 2-s2.0-84963593778 | |
dc.identifier.wos | 000365283800010 | |
dc.type.version | publishedVersion | en |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/6082/document.pdf | |
dc.citation.rank | M21 |