Alteration of the Activities of Trypsin and Leucine Aminopeptidase in Gypsy Moth Caterpillars Exposed to Dietary Cadmium
2015
Autori:
Vlahović, MilenaIlijin, Larisa
Mrdaković, Marija
Grčić, Anja
Matić, Dragana
Lazarević, Jelica
Perić Mataruga, Vesna
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2015, Springer International Publishing Switzerland
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
This paper examined the gut digestive enzymes, trypsin and leucine
aminopeptidase (LAP), in gypsy moth (Lymantria dispar, L.) larvae
exposed to cadmium. We analyzed the 3-day acute effect, chronic effects
from hatching until sacrifice, and recovery from long-term dietary
treatment with cadmium concentrations of 10 and 30 μg Cd/g dry food.
The activities of both examined enzymes declined at the higher level of
cadmium after both acute and chronic treatments and did not recover
within 3 days of feeding a diet with no added cadmium. Leucine
aminopeptidase was more sensitive because its activity was inhibited
after both short-term treatments. Three trypsin and one leucine
aminopeptidase isoform were detected by electrophoresis. Egg hatches
(full-sib families) differed in enzyme activities, index of phenotypic
plasticity, and isozyme expression after different treatments.
Statistically significant positive correlations between these enzymes
pointed to common genetic regulation. Moreover, variances were higher
for the control group than for cadmium treatment groups implying that
these proteolytic enzymes did not participate directly in
detoxification. These results suggest that, with additional research to
discover the mechanisms of enzyme inhibition, trypsin and leucine
aminopeptidase might be used as biomarkers to indicate the severity of
gastrointestinal disease due to cadmium intake.
Ključne reči:
Dietary cadmium; Gypsy moth; Trypsin; Leucine aminopeptidase; BiomarkersIzvor:
Water Air and Soil Pollution, 2015, 226, 11, 387-400Finansiranje / projekti:
- Uticaj magnetnih polja i drugih sredinskih stresora na fiziološke odgovore i ponašanje različitih vrsta (RS-MESTD-Basic Research (BR or ON)-173027)
DOI: 10.1007/s11270-015-2651-8
ISSN: 0049-6979