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Extracellular ATP Selectively Upregulates Ecto-Nucleoside Triphosphate Diphosphohydrolase 2 and Ecto-5'-Nucleotidase by Rat Cortical Astrocytes In Vitro
dc.creator | Brisevac, Dusica | |
dc.creator | Adzic, Marija | |
dc.creator | Laketa, Danijela | |
dc.creator | Parabucki, Ana | |
dc.creator | Milosevic, Milena | |
dc.creator | Lavrnja, Irena | |
dc.creator | Bjelobaba, Ivana | |
dc.creator | Sevigny, Jean | |
dc.creator | Kipp, Markus | |
dc.creator | Nedeljkovic, Nadezda | |
dc.date.accessioned | 2016-05-23T11:00:55Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1559-1166 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2349 | |
dc.description.abstract | Extracellular ATP (eATP) acts as a danger-associated molecular pattern which induces reactive response of astrocytes after brain insult, including morphological remodeling of astrocytes, proliferation, chemotaxis, and release of proinflammatory cytokines. The responses induced by eATP are under control of ecto-nucleotidases, which catalyze sequential hydrolysis of ATP to adenosine. In the mammalian brain, ecto-nucleotidases comprise three enzyme families: ecto-nucleoside triphosphate diphosphohydrolases 1-3 (NTPDase1-3), ecto-nucleotide pyrophosphatase/phospodiesterases 1-3 (NPP1-3), and ecto-5'-nucleotidase (eN), which crucially determine ATP/adenosine ratio in the pericellular milieu. Altered expression of ecto-nucleotidases has been demonstrated in several experimental models of human brain dysfunctions. In the present study, we have explored the pattern of NTPDase1-3, NPP1-3, and eN expression by cultured cortical astrocytes challenged with 1 mmol/L ATP (eATP). At the transcriptional level, eATP upregulated expression of NTPDase1, NTPDase2, NPP2, and eN, while, at translational and functional levels, these were paralleled only by the induction of NTPDase2 and eN. Additionally, eATP altered membrane topology of eN, from clusters localized in membrane domains to continuous distribution along the cell membrane. Our results suggest that eATP, by upregulating NTPDase2 and eN and altering the enzyme membrane topology, affects local kinetics of ATP metabolism and signal transduction that may have important roles in the process related to inflammation and reactive gliosis. | en |
dc.description.sponsorship | Serbian Ministry for Education and Science Project {[}III41014]; DAAD; Serbian Ministry of Education and Science; DAAD - Scientific Internship in Germany for Undergraduates and Graduates from Serbia | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | Journal of Molecular Neuroscience | |
dc.subject | Reactive astrogliosis | |
dc.subject | Extracellular ATP | |
dc.subject | Ecto-nucleotidase | |
dc.subject | Ecto-5 `-nucleotidase/CD73 | |
dc.title | Extracellular ATP Selectively Upregulates Ecto-Nucleoside Triphosphate Diphosphohydrolase 2 and Ecto-5'-Nucleotidase by Rat Cortical Astrocytes In Vitro | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Бјелобаба, Ивана; Кипп, Маркус; Милосевиц, Милена; Парабуцки, Aна; Севигнy, Јеан; Лаврња, Ирена Ц.; Брисевац, Дусица; Недељковиц, Надезда; Aдзиц, Марија; Лакета, Данијела; | |
dc.citation.issue | 3 | |
dc.citation.volume | 57 | |
dc.identifier.doi | 10.1007/s12031-015-0601-y | |
dc.identifier.scopus | 2-s2.0-84944275519 | |
dc.identifier.wos | 000363037000017 | |
dc.citation.spage | 452 | |
dc.citation.epage | 462 | |
dc.type.version | publishedVersion | en |
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